A multi-parametric islet perifusion system within a microfluidic perifusion device.

Journal of Visualized Experiments : JoVE
Adeola F AdewolaJose Oberholzer

Abstract

A microfluidic islet perifusion device was developed for the assessment of dynamic insulin secretion of multiple islets and simultaneous fluorescence imaging of calcium influx and mitochondrial potential changes. The device consists of three layers: first layer contains an array of microscale wells (500 mum diameter and 150 mum depth) that help to immobilize the islets while exposed to flow and maximize the exposed surface area of the islets; the second layer contains a circular perifusion chamber (3 mm deep, 7 mm diameter); and the third layer contains an inlet-mixing channel that fans out before injection into the perifusion chamber (2 mm in width, 19 mm in length, and 500 mum in height) for optimizing the mixing efficiency prior to entering the perifusion chamber. The creation of various glucose gradients including a linear, bell shape, and square shapes also can be created in the microfluidic perifusion network and is demonstrated.

References

Feb 12, 2009·Lab on a Chip·Javeed Shaikh MohammedDavid T Eddington

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Citations

Dec 5, 2013·Journal of Visualized Experiments : JoVE·Joe Fu-Jiou LoJose Oberholzer
Jan 22, 2021·Diabetes·Aaron L GliebermanKevin Kit Parker

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Methods Mentioned

BETA
ELISA
fluorescence imaging
chip

Software Mentioned

LabView
SimplePCI

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