A multi-scale analysis of bull sperm methylome revealed both species peculiarities and conserved tissue-specific features

BMC Genomics
Jean-Philippe PerrierHélène Kiefer

Abstract

Spermatozoa have a remarkable epigenome in line with their degree of specialization, their unique nature and different requirements for successful fertilization. Accordingly, perturbations in the establishment of DNA methylation patterns during male germ cell differentiation have been associated with infertility in several species. While bull semen is widely used in artificial insemination, the literature describing DNA methylation in bull spermatozoa is still scarce. The purpose of this study was therefore to characterize the bull sperm methylome relative to both bovine somatic cells and the sperm of other mammals through a multiscale analysis. The quantification of DNA methylation at CCGG sites using luminometric methylation assay (LUMA) highlighted the undermethylation of bull sperm compared to the sperm of rams, stallions, mice, goats and men. Total blood cells displayed a similarly high level of methylation in bulls and rams, suggesting that undermethylation of the bovine genome was specific to sperm. Annotation of CCGG sites in different species revealed no striking bias in the distribution of genome features targeted by LUMA that could explain undermethylation of bull sperm. To map DNA methylation at a genome-wide scale,...Continue Reading

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Jun 12, 2020·Animal : an International Journal of Animal Bioscience·M GasselinH Jammes
Jun 14, 2019·Frontiers in Genetics·Jingyue Ellie DuanXiuchun Cindy Tian
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Mar 13, 2021·Frontiers in Genetics·Mengqi Wang, Eveline M Ibeagha-Awemu

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Methods Mentioned

BETA
immunoprecipitation
biopsies
RRBS
genotyping
methylation
PCR
MeDIP-chip
PCA

Software Mentioned

Pyromark Q24
EVOLUTION
R
LUMA
GenomeStudio
chip
DAVID Database f or Annotation , Visualization and Integrated ...
MethPrimer
Shell
MeDIP

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