A multi-scale approach to determine accurate elemental and isotopic ratios by nano-scale secondary ion mass spectrometry imaging

Rapid Communications in Mass Spectrometry : RCM
Pierre-Joseph HattonDelphine Derrien

Abstract

Nano-scale secondary ion mass spectrometry (NanoSIMS) is still hampered by a lack of appropriate calibration method for the quantification of elemental and isotopic ratios in heterogeneous materials such as soil samples. The potential of (13)C-(15)N-labeled density fractions of soil to calibrate the C/N, (13)C/(12)C and (15)N/(14)N ratios provided by NanoSIMS was evaluated. The spatial organization of soil particles found at the macro- and micro-scales were compared. The C/N, (13)C/(12)C and (15)N/(14)N ratios measured at the macroscopic scale from different density fractions using an elemental analyzer coupled to an isotope ratio mass spectrometer (EA/IRMS) were compared with the corresponding micro-scale NanoSIMS measurements. When the macro- and micro-scales patterns were similar, macroscopic scale measurements obtained by EA/IRMS and the corresponding NanoSIMS C/N and (15)N/(14)N ratios averaged per fraction were used to obtain correction equations. The correction method using the internal calibration procedure was compared with the traditional one using a single organic standard. It was demonstrated that the correction method using an internal calibration procedure was applicable for NanoSIMS images acquired on more than 5...Continue Reading

References

Jul 31, 2002·Applied and Environmental Microbiology·John B CliffDavid D Myrold
Jun 12, 2004·Science·I M Young, J W Crawford
Jun 6, 2006·Journal of the American Society for Mass Spectrometry·G McMahonC J Unkefer
Nov 30, 2006·Rapid Communications in Mass Spectrometry : RCM·Anke M HerrmannDaniel V Murphy
Jan 12, 2008·Environmental Science & Technology·André J SimpsonBrian P Kelleher
Mar 1, 2012·Environmental Science & Technology·Laurent RemusatDelphine Derrien

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Citations

Jan 9, 2014·Nature Communications·Cordula VogelIngrid Kögel-Knabner
Feb 15, 2016·The Science of the Total Environment·Andrew H Bingham, M Francesca Cotrufo
Jul 15, 2015·Journal of Plant Nutrition and Soil Science = Zeitschrift Für Pflanzenernährung Und Bodenkunde·Jens KrusePeter Leinweber
Jul 22, 2015·Environmental Science & Technology·Carmen HöschenIngrid Kögel-Knabner
Dec 12, 2018·Environmental Science & Technology·Steffen SchlüterCarsten W Mueller

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