PMID: 9545096Apr 17, 1998Paper

A multicenter investigation with interphase fluorescence in situ hybridization using X- and Y-chromosome probes

American Journal of Medical Genetics
G W DewaldA Zinsmeister

Abstract

Twenty-six laboratories used X and Y chromosome probes and the same procedures to process and examine 15,600 metaphases and 49,400 interphases from Phaseolus vulgaris-leucoagglutinin (PHA)-stimulated lymphocytes. In Part I, each laboratory scored 50 metaphases and 200 interphases from a normal male and a normal female from its own practice. In Part II, each laboratory scored 50 metaphases and 200 interphases on slides prepared by a central laboratory from a normal male and a normal female and three mixtures of cells from the male and female. In Part III, each laboratory scored 50 metaphases (in samples of 5, 10, 15, and 20) and 100 interphases (in samples of 5, 10, 15, 20, and 50) on new, coded slides of the same specimens used in Part II. Metaphases from male specimens were scored as 98-99% XY with no XX cells, and 97-98% of interphases were scored as XY with 0.04% XX cells. Metaphases from female specimens were scored as 96-97% XX with 0.03% XY cells, and 94-96% of interphases were scored as XX with 0.05% XY cells. Considering the data as a model for any probe used with fluorescence in situ hybridization (FISH), a statistical approach assessing the impact of analytical sensitivity on the numbers of observations required to as...Continue Reading

References

Jul 3, 1995·American Journal of Medical Genetics·M RobinowA Veghte
Feb 2, 1996·American Journal of Medical Genetics·J L SpurbeckS M Jalal
Oct 28, 1996·American Journal of Medical Genetics·G W DewaldJ F Yung

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Citations

Feb 28, 2007·Leukemia & Lymphoma·Guillermo J Ruiz-ArgüellesJavier Garcés-Eisele
Apr 29, 2006·The Journal of Molecular Diagnostics : JMD·Roland A VenturaReiner Siebert
Mar 27, 2007·The Journal of Molecular Diagnostics : JMD·Daynna J WolffUNKNOWN American College of Medical Genetics Laboratory Quality Assurance Committee
Jan 25, 2006·Clinical and Laboratory Haematology·A L LobashevskyJ M Thomas
Aug 19, 2010·The Australian & New Zealand Journal of Obstetrics & Gynaecology·Kirralee PattonNarelle Hadlow
Jun 15, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Gábor PajorDonát Alpár
Apr 6, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Gabor PajorNorbert Sule
Jun 20, 2015·Prenatal Diagnosis·Michael T MennutiLorraine Dugoff
May 26, 2005·Journal of Clinical Forensic Medicine·F Mohammed, S M Tayel
Nov 22, 2014·American Journal of Medical Genetics. Part a·Paulie PapavassiliouColleen Jackson-Cook
Feb 20, 2010·São Paulo Medical Journal = Revista Paulista De Medicina·Azulamara da Silva RuizJosé Salvador Rodrigues de Oliveira
Jan 19, 2006·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Anne E WiktorGordon W Dewald
Aug 10, 1999·Luminescence : the Journal of Biological and Chemical Luminescence·P E Stanley, L J Kricka
May 8, 2001·Genetics in Medicine : Official Journal of the American College of Medical Genetics·UNKNOWN Test and Technology Transfer Committee, American College of Medical Genetics, 9650 Rockville Pike, Bethesda, MD 20814-3998,
Jul 9, 2011·Genetics in Medicine : Official Journal of the American College of Medical Genetics·James T MascarelloUNKNOWN Working Group of the American College of Medical Genetics Laboratory Quality Assurance Committee
Mar 10, 2005·Journal of Clinical Laboratory Analysis·Roger S RileyJonathan M Ben-Ezra
Jul 12, 2005·American Journal of Medical Genetics. Part a·Robert WallersteinBeth Pletcher
Jun 4, 2004·American Journal of Pharmacogenomics : Genomics-related Research in Drug Development and Clinical Practice·Christian Thiede
Jan 11, 2020·Current Protocols in Human Genetics·Susan M Zneimer

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