A multicolor flow cytometric assay for measurement of platelet-derived microparticles

Thrombosis Research
Fariborz MobarrezHåkan Wallén

Abstract

Flow cytometry (FCM) is the most commonly used method for detection of platelet-derived microparticles (PDMPs), but it is poorly standardized and mainly used for "bedside" analyses in fresh samples. If PDMPs could be analyzed in previously frozen samples it would increase the usefulness of the method. However, cell membrane fragments from contaminating cells created during freezing/thawing may cause artifacts and disturb measurements. PDMPs were labeled with monoclonal antibodies directed against CD42a and CD62P, or CD42a and CD142. The PDMP gate was determined using forward scatter (FSC) and CD42a expression. The mean fluorescence intensities (MFIs) of CD62P or CD142 positive particles were translated into MESF -values (Molecules of Equivalent Soluble Fluorochrome) using a standard curve. FITC-labeled phalloidin (which binds to intracellular actin) was used to detect destroyed cells/cell fragments. Phalloidin-positive particles were significantly more common in supernatants of frozen/thawed platelet rich and platelet poor plasma samples compared with supernatants of platelet free plasma. High-speed centrifugation was then used to obtain PDMP samples with low contamination of cell fragments. Electron microscopy showed that thes...Continue Reading

References

Aug 10, 1999·Critical Reviews in Oncology/hematology·L L Horstman, Y S Ahn
Aug 28, 2002·Diabetes·Florence SabatierFrancoise Dignat-George
May 14, 2003·The Journal of Cell Biology·Kurt L BarkalowJohn H Hartwig
Aug 12, 2003·Cardiovascular Research·Marja J VanWijkR Nieuwland
Feb 10, 2006·Blood Cells, Molecules & Diseases·Robert Flaumenhaft
Mar 1, 2007·Diabetes Care·Katarina Eeg-OlofssonUNKNOWN Steering Committee of the Swedish National Diabetes Register
Aug 12, 2008·Thrombosis Research·Françoise Dignat George
Mar 12, 2009·Thrombosis and Haemostasis·Laurent BurnierAnne Angelillo-Scherrer
May 22, 2009·Journal of Neurochemistry·Loïc DoeuvreEduardo Anglés-Cano
Sep 10, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Raj S KasthuriNigel Mackman

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Citations

Aug 13, 2014·Indian Journal of Hematology & Blood Transfusion : an Official Journal of Indian Society of Hematology and Blood Transfusion·Fatemeh YariReza Shiri
Sep 26, 2014·American Journal of Veterinary Research·Nora L SpringerTracy Stokol
Jan 1, 2012·Scientifica·Saulius Butenas
Mar 10, 2012·Rheumatology·David S PisetskyTony C Ning
Feb 1, 2013·American Journal of Veterinary Research·Sarah E HelmondMarjory B Brooks
Mar 5, 2013·Scandinavian Journal of Clinical and Laboratory Investigation·Christina ChristerssonAgneta Siegbahn
Apr 8, 2015·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Heather C InglisPhilip J Norris
Jun 26, 2012·Thrombosis Research·Manjunath Goolyam BasavarajJohn-Bjarne Hansen
Jul 20, 2010·Thrombosis Research·Sara TehraniGun Jörneskog
May 25, 2010·Biochemical and Biophysical Research Communications·Margareta Blombäck
Nov 18, 2015·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Jean Amiral, Jerard Seghatchian
Jan 16, 2016·BioMed Research International·Lotte Hatting PugholmMalene Møller Jørgensen
Feb 28, 2014·Thrombosis and Haemostasis·S BadrnyaA Assinger
May 23, 2015·Thrombosis and Haemostasis·T VajenR R Koenen
Mar 1, 2012·Thrombosis and Haemostasis·Mika SkeppholmHåkan Wallén
Feb 10, 2011·British Journal of Haematology·Sharon ZahraChristopher A Ludlam
May 16, 2013·Scandinavian Journal of Immunology·J R DyeD S Pisetsky
Dec 7, 2013·Journal of Cellular and Molecular Medicine·Isabel BochmannBurkhardt Dahlmann
Feb 24, 2015·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Micah J Mooberry, Nigel S Key
Jun 5, 2014·BioMed Research International·Jae-Lim ChoiJin-Yeong Han
Jan 5, 2011·Thrombosis Research·Pia R M Siljander
Jun 24, 2014·Clinical Therapeutics·Anne Louise Schacht RevenfeldMalene Jørgensen
May 3, 2016·British Journal of Haematology·Robert P Hebbel, Nigel S Key
Sep 24, 2015·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Tora AlmquistPaul Hjemdahl
Aug 18, 2016·Pathophysiology : the Official Journal of the International Society for Pathophysiology·J Winny YunJ Steven Alexander
Sep 3, 2016·Biomarkers in Medicine·Åsa ThulinAgneta Siegbahn
Nov 2, 2016·Clinical Chemistry and Laboratory Medicine : CCLM·Anna C SöderströmPernille J Vinholt
Nov 26, 2015·Cytometry. Part B, Clinical Cytometry·Wayne L Chandler
Dec 10, 2016·PloS One·Marta de Almeida SantiagoAna Cristina Martins de Almeida Nogueira

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