PMID: 374595Jan 1, 1979Paper

A multidimensional slit-scan flow system

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
J L CambierL L Wheeless

Abstract

A new slit-scan type flow system is described which provides three (X, Y, and Z) orthogonal one-dimensional projections of cell fluorescence. A photomultiplier tube and two semiconductor array detectors are used to obtain the three slit-scan contours from cells traversing a single fluorescence excitation beam. A high speed, dedicated preprocessor analyzes the three contours in parallel, extracting certain features useful for rejecting cells from which an accurate measurement of nuclear fluorescence cannot be obtain. Contour data is buffered and transferred to a PDP-11/40 computer where nuclear fluorescence is measured and cells are classified. It is anticipated that this new instrument will provide a significant reduction in false alarm rate when applied to prescreening of gynecologic cytology specimens.

Citations

Feb 15, 1988·Clinica Chimica Acta; International Journal of Clinical Chemistry·D H RyanP K Horan
Jul 31, 2007·Clinics in Laboratory Medicine·David A BasijiPhilip Morrissey
Feb 1, 1982·IEEE Transactions on Bio-medical Engineering·D B KayC L Brooks
Feb 1, 1983·Journal of Cutaneous Pathology·O P Clausen
Aug 1, 2015·Journal of Immunological Methods·David Basiji, Maurice R G O'Gorman
Jul 1, 1988·Cytometry·C CoxL L Wheeless
Nov 11, 2016·Lab on a Chip·Yuanyuan HanYu-Hwa Lo
Jul 1, 1989·Cytometry·R D RobinsonL L Wheeless
Jul 1, 1982·Cytometry·L A Weller, L L Wheeless
Sep 1, 1988·Cytometry·D M WheelessL L Wheeless
Jun 24, 2005·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Shiva MurthiDavid W Galbraith
Jun 3, 2020·Advanced Biosystems·Suwan N Jayasinghe
Apr 22, 2020·Nature Nanotechnology·Lucien E WeissYoav Shechtman
May 26, 2021·Cytometry. Part B, Clinical Cytometry·Jason StanleyKathy Fuller

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