A multifunctional human Argonaute2-specific monoclonal antibody.

RNA
Sabine RüdelGunter Meister

Abstract

Small regulatory RNAs including small interfering RNAs (siRNAs), microRNAs (miRNAs), or Piwi interacting RNAs (piRNAs) guide regulation of gene expression in many different organisms. The Argonaute (Ago) protein family constitutes the cellular binding partners of such small RNAs and regulates gene expression on the levels of transcription, mRNA stability, or translation. Due to the lack of highly specific and potent monoclonal antibodies directed against the different Ago proteins, biochemical analyses such as Ago complex purification and characterization rely on overexpression of tagged Ago proteins. Here, we report the generation and functional characterization of a highly specific monoclonal anti-Ago2 antibody termed anti-Ago2(11A9). We show that anti-Ago2(11A9) is specific for human Ago2 and detects Ago2 in Western blots as well as in immunoprecipitation experiments. We further demonstrate that Ago2 can be efficiently eluted from our antibody by a competing peptide. Finally, we show that anti-Ago2(11A9) recognizes Ago2 in immunofluorescence experiments, and we find that Ago2 not only localizes to cytoplasmic processing bodies (P-bodies) and the diffuse cytoplasm but also to the nucleus. With the anti-Ago2(11A9) antibody we ...Continue Reading

References

Oct 27, 2001·Science·M Lagos-QuintanaT Tuschl
Mar 27, 2002·Genes & Development·Zissimos MourelatosGideon Dreyfuss
Sep 17, 2002·Cell·Javier MartinezThomas Tuschl
Jul 21, 2004·Molecular Cell·Gunter MeisterThomas Tuschl
Jul 31, 2004·Science·Ji-Joon SongLeemor Joshua-Tor
Jul 31, 2004·Science·Jidong LiuGregory J Hannon
Aug 7, 2004·Science·Kevin V MorrisDavid J Looney
Sep 17, 2004·Nature·Gunter Meister, Thomas Tuschl
Jan 12, 2005·Nature Structural & Molecular Biology·G Brett RobbTariq M Rana
May 24, 2005·Nature Cell Biology·George L Sen, Helen M Blau
Jun 1, 2005·Current Opinion in Structural Biology·Witold FilipowiczRamesh S Pillai
Jun 7, 2005·Nature Cell Biology·Jidong LiuRoy Parker
Jun 24, 2005·Nature·Thimmaiah P ChendrimadaRamin Shiekhattar
Aug 6, 2005·Science·Ramesh S PillaiWitold Filipowicz
Sep 6, 2005·Science·Phillip D Zamore, Benjamin Haley
Oct 12, 2005·Structure·Traci M Tanaka Hall
Nov 8, 2005·Cell·Richard I GregoryRamin Shiekhattar
Nov 15, 2005·Nature Cell Biology·Andrew JakymiwEdward K L Chan
Nov 15, 2005·Nature Cell Biology·Jidong LiuGregory J Hannon
Nov 18, 2005·Current Biology : CB·Gunter MeisterThomas Tuschl
Dec 13, 2005·Biological Chemistry·Po Yu Chen, Gunter Meister
Dec 17, 2005·Genes & Development·Elisavet Maniataki, Zissimos Mourelatos
Jan 21, 2006·The EMBO Journal·Yoontae LeeV Narry Kim
Jan 26, 2006·Current Opinion in Structural Biology·Ji-Joon Song, Leemor Joshua-Tor
Jan 28, 2006·EMBO Reports·Philipp J F LeuschnerJavier Martinez
Feb 18, 2006·Science·Antonio J GiraldezAlexander F Schier
Jun 17, 2006·Cell·Suvendra N BhattacharyyaWitold Filipowicz
Jul 4, 2006·Genes & Development·Isabelle Behm-AnsmantElisa Izaurralde
Aug 29, 2006·Nature Structural & Molecular Biology·Daniel H KimJohn J Rossi
Aug 29, 2006·Nature Structural & Molecular Biology·Bethany A JanowskiDavid R Corey
Oct 13, 2006·Trends in Biochemical Sciences·James S Parker, David Barford
Nov 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Anthony K L LeungPhillip A Sharp
Dec 22, 2006·Nature Reviews. Molecular Cell Biology·Ana EulalioElisa Izaurralde
Jan 2, 2007·Trends in Cell Biology·Ramesh S PillaiWitold Filipowicz
Mar 27, 2007·Cold Spring Harbor Symposia on Quantitative Biology·D J PatelT Tuschl
Apr 4, 2007·Molecular and Cellular Biology·Ana EulalioElisa Izaurralde
May 29, 2007·Molecular Cell·G Brett Robb, Tariq M Rana
Jun 15, 2007·Molecular Cell·Lasse Peters, Gunter Meister
Jun 27, 2007·RNA·George EasowStephen M Cohen
Jul 20, 2007·RNA Biology·Michaela BeitzingerGunter Meister

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Citations

Nov 8, 2013·Functional & Integrative Genomics·Sadegh Azimzadeh JamalkandiAli Masoudi-Nejad
Apr 30, 2014·Nature Reviews. Genetics·Kevin V Morris, John S Mattick
Apr 1, 2014·The International Journal of Biochemistry & Cell Biology·Marika SalmanidisCameron Bracken
Aug 20, 2014·Molecular Therapy. Nucleic Acids·Thomas C Roberts
Aug 23, 2008·RNA·Yohei Kirino, Zissimos Mourelatos
Aug 27, 2009·BMC Research Notes·Yukinobu HayashidaTsutomu Kurokawa
Oct 8, 2009·Nucleic Acids Research·Grace S TanMarianthi Kiriakidou
Oct 12, 2010·RNA·Alessandra MescalchinGeorg Sczakiel
Nov 13, 2010·Nucleic Acids Research·Sabine RüdelGunter Meister
Feb 16, 2011·Nucleic Acids Research·Xue-Hai Liang, Stanley T Crooke
Oct 15, 2011·Antioxidants & Redox Signaling·Jin Hwa JangSoo Kyung Kang
Sep 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Janina PfaffGunter Meister
Mar 18, 2009·Virus Genes·Lars DölkenUlrich H Koszinowski
Jul 31, 2012·Nucleic Acids Research·Anne DueckGunter Meister
Nov 13, 2012·Nature Cell Biology·Derrick GibbingsOlivier Voinnet
Sep 10, 2011·Antioxidants & Redox Signaling·Jin Hwa JangSoo Kyung Kang
Jul 28, 2010·Nature Reviews. Genetics·Jacek KrolWitold Filipowicz
Jun 5, 2013·Nature Reviews. Genetics·Gunter Meister
Jul 31, 2013·Genome Biology·Florian ErhardRalf Zimmer
Feb 9, 2016·Cell Reports·Sunita ShankarArul M Chinnaiyan
Nov 28, 2015·Nucleic Acids Research·Roya KalantariDavid R Corey
Sep 10, 2015·Proceedings of the National Academy of Sciences of the United States of America·Judith HauptmannGunter Meister
Oct 2, 2012·Methods : a Companion to Methods in Enzymology·Lukasz JaskiewiczMihaela Zavolan
Mar 27, 2012·Biochimie·Michèle Ernoult-LangeDominique Weil
Apr 25, 2009·Advanced Drug Delivery Reviews·Donald D RaoJohn Nemunaitis
Aug 23, 2011·The EMBO Journal·Daniel SchraivogelGunter Meister
Aug 9, 2011·Wiley Interdisciplinary Reviews. RNA·Victoria PortnoyLong-Cheng Li
Mar 21, 2013·The EMBO Journal·Lukas StalderNicole C Meisner-Kober
Apr 10, 2016·Nucleic Acids Research·Marc S Weinberg, Kevin V Morris
Sep 27, 2014·Wiley Interdisciplinary Reviews. RNA·Eric Batsché, Maya Ameyar-Zazoua
May 31, 2014·Nucleic Acids Research·Michael HannusGunter Meister

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