A multigene family encoding R-SNAREs in the ciliate Paramecium tetraurelia

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Christina SchildeRoland Kissmehl

Abstract

SNARE proteins (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) mediate membrane interactions and are conventionally divided into Q-SNAREs and R-SNAREs according to the possession of a glutamine or arginine residue at the core of their SNARE domain. Here, we describe a set of R-SNAREs from the ciliate Paramecium tetraurelia consisting of seven families encoded by 12 genes that are expressed simultaneously. The complexity of the endomembrane system in Paramecium can explain this high number of genes. All P. tetraurelia synaptobrevins (PtSybs) possess a SNARE domain and show homology to the Longin family of R-SNAREs such as Ykt6, Sec22 and tetanus toxin-insensitive VAMP (TI-VAMP). We localized four exemplary PtSyb subfamilies with GFP constructs and antibodies on the light and electron microscopic level. PtSyb1-1, PtSyb1-2 and PtSyb3-1 were found in the endoplasmic reticulum, whereas PtSyb2 is localized exclusively in the contractile vacuole complex. PtSyb6 was found cytosolic but also resides in regularly arranged structures at the cell cortex (parasomal sacs), the cytoproct and oral apparatus, probably representing endocytotic compartments. With gene silencing, we showed that the R-SNARE of the contracti...Continue Reading

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Citations

Jun 17, 2008·Eukaryotic Cell·Christina SchildeHelmut Plattner
Mar 26, 2011·PloS One·Paul N UlrichRoberto Docampo
Oct 7, 2008·The International Journal of Biochemistry & Cell Biology·Joel B DacksMark C Field
Mar 3, 2007·Trends in Cell Biology·Nels C EldeAaron P Turkewitz
Nov 17, 2007·Biology of the Cell·Eri NishiharaSeiji Sonobe
Aug 8, 2013·Critical Reviews in Microbiology·Helmut Plattner
May 25, 2013·PloS One·David W MorgensAndre R O Cavalcanti
Feb 4, 2011·Trends in Cell Biology·Sean R Collins, Tobias Meyer
Oct 22, 2015·Biological Reviews of the Cambridge Philosophical Society·Helmut Plattner
Jul 19, 2017·The Journal of Eukaryotic Microbiology·Helmut Plattner
Mar 20, 2012·Journal of Cell Science·Karin Komsic-BuchmannBurkhard Becker
Aug 24, 2007·Journal of Cell Science·Joel B Dacks, Mark C Field

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