PMID: 8994162Dec 1, 1996Paper

A multiple wavelength algorithm in color image analysis and its applications in stain decomposition in microscopy images

Medical Physics
R ZhouD L Parker

Abstract

Stains have been used in optical microscopy to visualize the distribution and intensity of substances to which they are attached. Quantitative measures of optical density in the microscopic images can in principle be used to determine the amount of the stain. When multiple dyes are used to simultaneously visualize several substances to which they are specifically attached, quantification of each stain cannot be made using any single wavelength because attenuation from the several stain components contributes to the total optical density. Although various dyes used as optical stains are perceived as specific colors, they, in fact, have complex attenuation spectra. In this paper, we present a technique for multiple wavelength image acquisition and spectral decomposition based upon the Lambert-Beer absorption law. This algorithm is implemented based on the different spectral properties of the various stain components. By using images captured at N wavelengths, N components with different colors can be separated. This algorithm is applied to microscopy images of doubly and triply labeled prostate tissue sections. Possible applications are discussed.

Citations

Jul 7, 2009·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Vagner BernardoUlysses Lins
Sep 1, 2008·Expert Opinion on Medical Diagnostics·Richard M LevensonMassimo Loda
Oct 19, 2004·Journal of Biomedical Informatics·Tim W Nattkemper
Feb 13, 2001·The American Journal of Pathology·L ErmertM Ermert
Dec 2, 2015·IEEE Journal of Biomedical and Health Informatics·Xingyu Li, Konstantinos N Plataniotis
Mar 7, 2006·The Annals of Otology, Rhinology, and Laryngology·Mariah S HahnRobert Langer
Sep 18, 1999·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R L OrnbergD A Edwards
May 9, 2006·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Richard M Levenson
Sep 14, 2006·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Yuval GariniGeorge McNamara
Oct 1, 2020·Bioinformatics·Gabriel LandiniFilippo Piccinini

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.