A multiplex paper-based nanobiocatalytic system for simultaneous determination of glucose and uric acid in whole blood

The Analyst
Jin HuangJianhui Jiang

Abstract

In this work, a versatile point-of-care assay platform based on a microfluidic paper-based analytic device (μPAD) was developed for the simultaneous detection of multiple targets. The μPAD with a central zone and six test zones is fabricated by a simple and inexpensive wax printing method. A flower-like hybrid nanocomplex synthesized with specific dual enzymes and Cu3(PO4)2 inorganic nanocrystals is spotted in the test zones on the μPAD, followed by the introduction of assay targets. Using dual-enzyme inorganic hybrid nanoflowers in the μPAD as nanobiocatalysts, which preserve the activity and enhance the stability of the enzymes, based on the H2O2-mediated catalytic oxidizing chromogenic reaction produced by glucose/uric acid, the developed multiplex paper-based nanobiocatalytic system is demonstrated to enable simultaneous and sensitive detection of glucose and uric acid with a detection limit of 60 and 25 μM, respectively. More importantly, it has been successfully used for detecting glucose and uric acid levels in human whole blood samples. The developed multiplex paper-based nanobiocatalytic system features very easy fabrication and operation, low cost, and high sensitivity and has promising prospects for a clinical multia...Continue Reading

References

Mar 18, 2004·Current Medical Research and Opinion·Michael Alderman, Kala J V Aiyer
Aug 24, 2006·Journal of the American Chemical Society·Ming YanPingkai Ouyang
Dec 28, 2006·Cleveland Clinic Journal of Medicine·Marcelo Heinig, Richard J Johnson
Mar 25, 2008·Annual Review of Biomedical Engineering·Paul YagerJohn Gerdes
Dec 10, 2008·Proceedings of the National Academy of Sciences of the United States of America·Andres W MartinezGeorge M Whitesides
May 1, 2009·Nature Reviews. Drug Discovery·Nigel A CalcuttAnn Marie Schmidt
Oct 28, 2009·ACS Nano·David R Walt
Aug 4, 2010·Analytica Chimica Acta·Wijitar DungchaiCharles S Henry
Jan 10, 2012·Analytical Chemistry·Vladimir GubalaDavid E Williams
Jun 5, 2012·Nature Nanotechnology·Jun GeRichard N Zare
Oct 4, 2012·Chemical Society Reviews·Claudio Parolo, Arben Merkoçi
Jan 16, 2013·Journal of the American Chemical Society·Liang-Bing WangJ G Hou
Feb 21, 2013·Chemistry, an Asian Journal·Lin ZhuRichard N Zare
May 9, 2013·Lab on a Chip·Ali Kemal YetisenChristopher R Lowe
Nov 20, 2013·Accounts of Chemical Research·Kangning RenHongkai Wu
Sep 6, 2014·Chemical Communications : Chem Comm·Zhixian LiZheng Liu
Apr 14, 2015·Angewandte Chemie·Kentaro YamadaDaniel Citterio
Apr 29, 2015·Angewandte Chemie·Ashok A KumarGeorge M Whitesides

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Citations

Mar 19, 2021·Critical Reviews in Biotechnology·Shamraja S NadarDinesh J Ahirrao
Apr 22, 2021·Lab on a Chip·Wen-Chin LeeLung-Ming Fu
Jul 2, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Xiao-Yang ZhaoDao-Kuan Jiang

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Methods Mentioned

BETA
biosensor
biosensors
biosensing
X-ray
chips

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Adobe
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