A multiplex PCR for beta-lactamase genes of Haemophilus influenzae and description of a new blaTEM promoter variant

The Journal of Antimicrobial Chemotherapy
Stephen G Tristram, Scott Nichols

Abstract

To establish a multiplex PCR to detect and differentiate the beta-lactamase genes of Haemophilus influenzae. A collection of H. influenzae isolates with a range of different beta-lactamase genes were tested in parallel with individual PCRs and the multiplex assay developed in this study. The multiplex method was found to be superior to previous methods and was able to correctly detect and differentiate blaROB-1 and the variants of blaTEM present in the strains tested, including a previously unrecognized variant associated with a 54 bp insertion in the promoter region. The multiplex PCR will be a useful tool for the surveillance of beta-lactamase genes in H. influenzae.

Citations

Jul 12, 2012·The Journal of Antimicrobial Chemotherapy·Stephen G TristramGareth L Harvey
Jun 27, 2012·Antimicrobial Agents and Chemotherapy·Annette SøndergaardNiels Nørskov-Lauritsen
Apr 13, 2007·Clinical Microbiology Reviews·Stephen TristramPeter C Appelbaum
Sep 27, 2012·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Chong LuoYu Shi
May 17, 2011·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Niels Nørskov-LauritsenKurt Fuursted
May 15, 2018·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A Aguirre-QuiñoneroL Martínez-Martínez
Feb 14, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Nambram Somendro SinghJugsharan Singh Virdi

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