A multiplex PCR procedure for polymorphic analysis of GSTM1 and GSTT1 genes in population studies

Cancer Letters
S Z Abdel-RahmanW W Au

Abstract

A deletion polymorphism in glutathione S-transferase theta (GSTT1) gene was recently discovered in humans. Similar to the GSTM1 gene, GSTT1 is also recognized as a risk modifier in exposed populations. To evaluate the role of genetic polymorphism in health effects, the combined genetic polymorphism of different genes should be taken into consideration. In the present study, we have developed a multiplex PCR approach for simultaneous replication of both genes for molecular analysis. The multiplex PCR protocol was validated using donor DNA with different polymorphic combinations for both genes from two different ethnic populations (North Americans and Egyptians). The prevalence of the GSTM1 null genotype was 51% among North Americans and 44% among Egyptians. The prevalence of the GSTT1 null genotype was 15% among North Americans and 14.7% among Egyptians. Combined polymorphism analysis of both genes revealed that 6.3% of North Americans harbor the deleted genotype of both genes compared to 8.8% of the Egyptians. The data indicate that there is no major difference in allelic distribution of both genes between the ethnic populations. The multiplex PCR assay used in this study has the advantage of reducing the time, effort and cost ...Continue Reading

References

Nov 1, 1995·American Journal of Surgery·Z TriznaH Goepfert
Jan 1, 1995·Journal of Occupational and Environmental Medicine·A Hirvonen
Aug 1, 1994·Journal of Biochemical Toxicology·S Z Abdel-RahmanA E Ahmed

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Citations

Jun 3, 2010·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Aysun TürkanoğluOrhan Adali
Jun 10, 2011·Molecular and Cellular Biochemistry·Renato PolimantiMaria Fuciarelli
Jun 22, 2010·Molecular Biology Reports·Sara PiacentiniMaria Fuciarelli
Sep 18, 2007·Journal of Molecular Neuroscience : MN·Kyung-Suk MoonJae-Young Um
Dec 4, 2003·Mutation Research·Luis Felipe Ribeiro PintoNelson Adami Andreollo
Aug 15, 2002·Cancer Letters·Lídia da Fonte de AmorimLuis Pinto
Jun 4, 1999·Mutation Research·M TaningherS Parodi
Sep 8, 2010·Mutagenesis·Domenico PalliEugenia Dogliotti
Jan 5, 2011·Mutagenesis·W A García-SuásteguiM E Gonsebatt
Oct 5, 2012·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Sonja SuvakovTatjana Simic
Jan 10, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Nur DualeGunnar Brunborg
Nov 13, 2004·Applied Immunohistochemistry & Molecular Morphology : AIMM·Abida K HaqueJingwu Xie
Jun 26, 2010·Pharmacogenetics and Genomics·Carolina Salinas-SouzaSilvia R C de Toledo
Sep 21, 2007·Clinical and Experimental Pharmacology & Physiology·Anahita Torkaman-BoutorabiIraj Milanian
Jul 30, 2009·Journal of Gastroenterology and Hepatology·Shouji Shimoyama
Sep 5, 2008·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Mala PandeMarsha L Frazier
Jun 14, 2013·BMC Medical Genetics·Fernando Augusto de Lima MarsonJosé Dirceu Ribeiro
Oct 1, 2009·Genetics and Molecular Biology·Marcos Euzébio MacielEnilze Maria de Souza Fonseca Ribeiro
Jun 3, 2011·The Oncologist·Maarten J DeenenJan H M Schellens
Jun 11, 2011·The Oncologist·Maarten J DeenenJan H M Schellens
Oct 20, 2010·Molecular Medicine·Jeffrey K WickliffeKarl E Anderson
Mar 7, 2014·BMC Medical Genetics·Fernando Augusto de Lima MarsonJose Dirceu Ribeiro
Aug 3, 2012·Journal of Toxicology and Environmental Health. Part a·Gustavo Rafael Mazzaron BarcelosFernando Barbosa
Nov 28, 2013·Environmental Science and Pollution Research International·Fernanda Ribeiro GodoyDaniela de Melo E Silva
Jul 4, 2008·Archives of Toxicology·Maria FuciarelliFlavia Porreca
Apr 17, 1998·Cancer Detection and Prevention·S Z Abdel-RahmanW W Au
Nov 4, 2008·Clinical and Experimental Medicine·João Paulo Souto GrandoIlce Mara de Syllos Cólus
Jun 23, 2009·International Journal of Colorectal Disease·Davy Carlos Mendes RapozoLuiz Felipe Ribeiro Pinto
Aug 25, 2012·Journal of Nucleic Acids·Priti SharmaSudhir K Goel
Aug 10, 2011·The Science of the Total Environment·Shiv Kumar GiriSanjeev Kumar Gautam

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