A multiplex real-time polymerase chain reaction for simultaneous detection of Salmonella spp., Escherichia coli O157, and Listeria monocytogenes in meat products

Foodborne Pathogens and Disease
Biao SuoXianming Shi

Abstract

To achieve an effective detection of Salmonella spp., Escherichia coli O157, and Listeria monocytogenes in meat products, a multiplex real-time polymerase chain reaction (PCR) coupled with a multipathogen enrichment strategy was developed in this study. Pathogen-specific DNA sequences in the invA, rfbE, and hlyA genes were employed to design primers and TaqMan probes for identifying Salmonella spp., E. coli O157, and L. monocytogenes, respectively. An internal amplification control (IAC) utilizing a novel DNA sequence from human adenovirus was incorporated into the multiplex PCR assay to indicate false-negative results. Concurrent amplifications of multiple targets and IAC were thoroughly evaluated and optimized to minimize PCR competitions. Combined with a multipathogen enrichment in a selective enrichment broth for Salmonella, Escherichia, and Listeria (SEL), the multiplex real-time PCR assay was able to simultaneously detect all of the three organisms in artificially contaminated ground beef at a detection sensitivity of <18 CFU/10 g ground beef. Applying the assay to 26 retail meat samples including beef, chicken, turkey, and pork revealed that 12 samples were positive for one of the organisms and 3 samples were positive fo...Continue Reading

References

Apr 1, 1991·Clinical Microbiology Reviews·A SchuchatC V Broome
May 26, 1999·Journal of Food Protection·K H SeoD P Campbell
Nov 15, 2002·Journal of Food Protection·Sophia Kathariou
Dec 10, 2003·Journal of Clinical Microbiology·Jeffrey HoorfarPatrick Fach
Mar 11, 2004·Clinical Chemistry·Siegfried Burggraf, Bernhard Olgemöller
Jan 19, 2006·Clinical Microbiology Reviews·M J EspyT F Smith
Jul 1, 1994·Journal of Food Protection·Phillip I Tarr

❮ Previous
Next ❯

Citations

Feb 6, 2013·Analytical and Bioanalytical Chemistry·Peter IrwinYiping He
Feb 4, 2011·Foodborne Pathogens and Disease·Beatriz QuiñonesErica D Dawson
Apr 5, 2012·Foodborne Pathogens and Disease·Giulia AmaglianiGiorgio Brandi
Apr 27, 2011·Journal of Perinatal Medicine·Ronald F LamontRoberto Romero
Apr 10, 2013·International Journal of Circumpolar Health·David M GoldfarbRobert Slinger
Jun 22, 2011·Annual Review of Analytical Chemistry·Andrew G Gehring, Shu-I Tu
May 17, 2011·Food Microbiology·Florence PostollecDanièle Sohier
Apr 11, 2015·New Biotechnology·Delfina BrandãoMaría Isabel Pividori
Jul 30, 2004·International Journal of Food Microbiology·Samantha Hu Liming, Arvind A Bhagwat
Nov 13, 2013·Journal of Food Protection·Abdela WoubitTemesgen Samuel
Jan 17, 2019·Critical Reviews in Analytical Chemistry·Shalini MuniandyBey Fen Leo
Feb 7, 2018·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Kristin A ClothierAshley E Hill
Feb 16, 2018·Current Environmental Health Reports·I R Lake, G C Barker
Jul 31, 2020·Biosensors & Bioelectronics·Maha AlafeefDipanjan Pan
Jan 9, 2020··Wan Maisarah Mukhtar, Wan Maisarah Mukhtar

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

BLAST
Primer
Lasergene
Primer Express
MegAlign

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.