A Multiplexed Cell-Based Assay for the Identification of Modulators of Pre-Membrane Processing as a Target against Dengue Virus

Journal of Biomolecular Screening
Zachary D StolpRoland Wolkowicz

Abstract

The DenV pre-membrane protein (prM) is a crucial chaperone for the viral envelope protein, preventing premature fusion with vesicles during viral export. prM molecules in immature particles are cleaved by host proteases, leading to mature fusogenic virions. Blockade of prM cleavage would restrict fusion and represents a novel druggable opportunity against DenV. We have thus established a cell-based platform to monitor prM processing that relies on an engineered two-tag scaffold that travels to the cell surface through the secretory pathway. The assay discriminates between a single cell-surface tag when prM is cleaved and two tags when it is not, as detected through fluorescent-coupled antibodies by flow cytometry. The assay, miniaturized into a 96-well plate format, was multiplexed with the HIV-1 envelope boundary, also cleaved in the same pathway. A pilot screen against 1280 compounds was executed, leading to the identification of a potential active and corroborating the robustness of our assay for large-scale screening. We describe for the first time a cell-based assay that monitors DenV prM processing within the classical secretory pathway, which was exploited to identify a potential novel drug against DenV.

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Citations

Sep 24, 2015·Future Medicinal Chemistry·Roland Wolkowicz
Jan 1, 2015·Biochemistry Insights·Darin AbbadessaRoland Wolkowicz
Oct 22, 2019·Systematic Biology·Michael R McGowenStephen J Rossiter
Jul 25, 2019·Frontiers in Cellular and Infection Microbiology·Megan ChesnutDavid Pamies
Jan 3, 2020·Current Medicinal Chemistry·Ting YangHailin Cong

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Methods Mentioned

BETA
biosensor
flow cytometry
Assay
Transfection
FACS
PCL

Software Mentioned

FACSDiva
FlowJo
DenV
CytoBank

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