A multistage mycobacterium tuberculosis subunit vaccine LT70 including latency antigen Rv2626c induces long-term protection against tuberculosis

Human Vaccines & Immunotherapeutics
Xun LiuBingdong Zhu

Abstract

To develop an effective subunit vaccine which could target tubercle bacilli with different metabolic states and provide effective protective immunity, we fused antigens ESAT6, Ag85B, peptide 190-198 of MPT64, and Mtb8.4 mainly expressed by proliferating bacteria and latency-associated antigen Rv2626c together to construct a multistage protein ESAT6-Ag85B-MPT64(190-198)-Mtb8.4-Rv2626c (LT70 for short) with the molecular weight of 70 kDa. The human T-cell responses to LT70 and other antigens were analyzed. The immune responses of LT70 in the adjuvant of DDA and Poly I:C and its protective efficacy against Mycobacterium tuberculosis (M. tuberculosis) infection in C57BL/6 mice were evaluated. The results showed that LT70 was stably produced in Escherichia coli and could be purified by successive salting-out and chromatography. LT70 could be strongly recognized by human T cells from TB patients and persons who are supposed latently infected with M. tuberculosis. The subunit vaccine LT70 generated strong antigen-specific humoral and cell-mediated immunity, and induced higher protective efficacy (5.41±0.37 Log10 CFU in lung) than traditional vaccine Bacillus Calmette-Guerin (6.01±0.33 Log10 CFU) and PBS control (6.53±0.26 Log10 CFU) a...Continue Reading

References

Jan 11, 1990·Annual Review of Immunology·F D FinkelmanW E Paul
Jun 22, 1999·The Journal of Experimental Medicine·S M BeharM B Brenner
Aug 26, 2000·Cytokine & Growth Factor Reviews·S E Dorman, S M Holland
May 9, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·R N ColerM R Alderson
Jan 25, 2002·The Journal of Infectious Diseases·Troels LillebaekAse B Andersen
Apr 26, 2002·Trends in Microbiology·Mark J Pallen
Dec 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Lanbo ShiRobert J North
Jul 14, 2005·Nature Reviews. Microbiology·Peter Andersen, T Mark Doherty
Sep 24, 2005·Infection and Immunity·Amy DavidowMaria L Gennaro
Dec 5, 2006·Trends in Microbiology·Peter Andersen
Sep 28, 2007·Clinical Pharmacology and Therapeutics·Y Zhang
Apr 22, 2009·PLoS Pathogens·Crystal Y ChenZheng W Chen
Apr 9, 2011·Nature Reviews. Immunology·Gerhard WalzlAlimuddin Zumla
Mar 28, 2012·Tuberculosis·Michael J Brennan, Jelle Thole
Jan 15, 2013·ChemMedChem·Peter Michael Moyle, Istvan Toth
Jan 22, 2013·Respirology : Official Journal of the Asian Pacific Society of Respirology·Charles C WangYing Zhang
May 28, 2013·Seminars in Immunology·Stefan H E Kaufmann
May 6, 2014·Frontiers in Immunology·Ana Paula Junqueira-KipnisAndré Kipnis
May 31, 2014·Trends in Immunology·Peter Andersen, Joshua S Woodworth
Jun 4, 2014·Cold Spring Harbor Perspectives in Medicine·Peter Andersen, Stefan H E Kaufmann
Jul 11, 2014·Frontiers in Immunology·Annemieke GelukTom H M Ottenhoff
Jul 24, 2015·Clinical and Vaccine Immunology : CVI·Mariateresa CoppolaAnnemieke Geluk

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Citations

Jan 18, 2018·Respirology : Official Journal of the Asian Pacific Society of Respirology·Bingdong ZhuYing Zhang
Jun 2, 2017·Frontiers in Microbiology·Huiyu ChenYing Zhang
Jun 6, 2018·International Journal of Environmental Research and Public Health·Yu HuYing Wang
Oct 9, 2020·Vaccines·E WhitlowS N M Hanif

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Methods Mentioned

BETA
ELISA
PCR
Electrophoresis
protein assay
enzyme-linked immunosorbent assay
light microscopy

Software Mentioned

SPSS13

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