A multistage testing strategy for detection of quantitative trait Loci affecting disease resistance in Atlantic salmon

Genetics
Thomas MoenL Gomez-Raya

Abstract

A multistage testing strategy to detect QTL for resistance to infectious salmon anemia (ISA) in Atlantic salmon is proposed. First, genotyping of amplified fragment length polymorphisms (AFLP) and a transmission disequilibrium test (TDT) were carried out using dead offspring from a disease resistance challenge test. Second, AFLP genotyping among survivors followed by a Mendelian segregation test was performed. Third, within-family survival analyses using all offspring were developed and applied to significant TDT markers with Mendelian inheritance. Maximum-likelihood methodology was developed for TDT with dominant markers to exploit linkage disequilibrium within families. The strategy was tested with two full-sib families of Atlantic salmon sired by the same male and consisting of 79 offspring in total. All dead offspring from the two families were typed for 64 primer combinations, resulting in 340 scored markers. There were 26 significant results out of 401 TDTs using dead offspring. In the second stage, only 17 marker families showed Mendelian segregation and were tested in survival analysis. A permutation test was performed for all survival analyses to compute experimentwise P-values. Two markers, aaccac356 and agccta150, we...Continue Reading

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Citations

Feb 26, 2008·Marine Biotechnology·E SarropoulouG Kotoulas
Apr 16, 2013·Veterinary Immunology and Immunopathology·Maaria KilpiEsa-Matti Lilius
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Apr 28, 2019·Cells·Takuya Yamaguchi, Johannes M Dijkstra

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