A Multiwell Cardiac μGMEA Platform for Action Potential Recordings from Human iPSC-Derived Cardiomyocyte Constructs

Stem Cell Reports
Stacie L EdwardsRosy Joshi-Mukherjee

Abstract

Multielectrode array (MEA) technology has been extensively used for field potential recordings from excitable cells. However, its application for action potential (AP) measurements has not been harnessed. Here, we report a novel platform for high-resolution intracellular AP recordings from induced pluripotent stem cell-cardiomyocyte constructs derived from human cardiac fibroblasts. To gain intracellular access, micro-gold MEAs were used to electroporate multiple constructs simultaneously. High-throughput AP measurements were obtained from 41 multicellular constructs. Repeated electroporations of the same cells did not affect the signal stability. Our model has the capability to distinguish subtle differences in AP morphology to characterize the network profile. Furthermore, we confirm the reliability of the system by recapitulating known drug-induced physiological and arrhythmogenic responses. Overall, the model provides a unique cardio-electronic interface for non-invasive measurements of AP dynamics for drug screening and disease modeling. This technology opens the door for identifying novel cardio-factors to enhance electrophysiological maturation.

Citations

Jan 17, 2020·Scientific Reports·Allison L BrillBarbara E Ehrlich
Mar 1, 2021·Journal of Electrocardiology·John JurkiewiczPeter Hinow

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Methods Mentioned

BETA
chips
flow cytometry
fluorescence-activated cell sorting
FACS
confocal

Software Mentioned

MATLAB Signal Processing and Statistics Machine Learning Toolb...
Multiwell
EVOS FL
pClamp10
- Analyzer Channel Systems MCS
μGMEA Construct
FACSDiva
Fluoview
MCS
μGMEA

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