A Murine Model of Candida glabrata Vaginitis Shows No Evidence of an Inflammatory Immunopathogenic Response

PloS One
Evelyn E NashP L Fidel

Abstract

Candida glabrata is the second most common organism isolated from women with vulvovaginal candidiasis (VVC), particularly in women with uncontrolled diabetes mellitus. However, mechanisms involved in the pathogenesis of C. glabrata-associated VVC are unknown and have not been studied at any depth in animal models. The objective of this study was to evaluate host responses to infection following efforts to optimize a murine model of C. glabrata VVC. For this, various designs were evaluated for consistent experimental vaginal colonization (i.e., type 1 and type 2 diabetic mice, exogenous estrogen, varying inocula, and co-infection with C. albicans). Upon model optimization, vaginal fungal burden and polymorphonuclear neutrophil (PMN) recruitment were assessed longitudinally over 21 days post-inoculation, together with vaginal concentrations of IL-1β, S100A8 alarmin, lactate dehydrogenase (LDH), and in vivo biofilm formation. Consistent and sustained vaginal colonization with C. glabrata was achieved in estrogenized streptozotocin-induced type 1 diabetic mice. Vaginal PMN infiltration was consistently low, with IL-1β, S100A8, and LDH concentrations similar to uninoculated mice. Biofilm formation was not detected in vivo, and co-in...Continue Reading

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Citations

May 20, 2017·Mycopathologia·Marcelo Ortega-RiverosElena Eraso
Mar 7, 2017·Research in Microbiology·Mathieu CauchieKatrien Lagrou
Oct 31, 2017·Drug Discoveries & Therapeutics·Yuto KochiChikara Kaito
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Nov 8, 2017·Infection and Immunity·Jonathan P RichardsonBrian M Peters
Dec 21, 2017·Scientific Reports·Elena RosellettiAnna Vecchiarelli
Jul 20, 2016·Infection and Immunity·Mary Ann Jabra-RizkMairi Noverr
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Jul 17, 2020·Expert Review of Anti-infective Therapy·Mubashshir RasheedRupinder Kaur

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Methods Mentioned

BETA
lavages
lavage
light microscopy
enzyme-linked immunosorbent assays
Assay
confocal microscopy
ELISA
enzyme-linked immunosorbent assay

Software Mentioned

Graph Pad
Fluoview
Prism

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