PMID: 7021158Jun 1, 1981Paper

A mutant elongation factor Tu which does not immobilize the ribosome upon binding of kirromycin

European Journal of Biochemistry
F J DuisterwinkelL Bosch

Abstract

In the accompanying paper we have shown that polypeptide synthesis sustained by the mutant elongation factor EF-TuBO is inhibited by kirromycin. Here we have searched for the primary site of inhibition in the elongation cycle. It is demonstrated that in the presence of the antibiotic EF-TuBO can form a complex with aminoacyl-tRNA and GTP and that the complex is able to bind to ribosomes programmed with poly(U). Like its wild-type counterpart, EF-TuBO . GDP can form a quaternary complex with aminoacyl-tRNA and kirromycin but, unlike the wild-type quaternary complex, the mutant complex fails to associate with the ribosome. This explains the recessive nature of the tuf B mutation in cells producing kirromycin-resistant EF-TuA and EF-TuBO. It also suggests a mechanism for the inhibition by kirromycin of EF-TuBO-dependent polypeptide synthesis.

References

Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·E FischerA Parmeggiani
Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J A Van de KlundertL Bosch
Sep 21, 1978·Biochimica Et Biophysica Acta·Y Kaziro
Dec 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·H WolfA Parmeggiani
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P H Van der MeideL Bosch
Jun 1, 1981·European Journal of Biochemistry·P H Van der MeideT Blumenthal

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Citations

Feb 1, 1983·European Journal of Biochemistry·P H van der MeideL Bosch
Feb 1, 1983·European Journal of Biochemistry·P H van der MeideL Bosch
Nov 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·B KraalR Hilgenfeld
Mar 10, 1990·European Journal of Biochemistry·S Tapio, L A Isaksson
Jun 1, 1981·European Journal of Biochemistry·P H Van der MeideT Blumenthal
Mar 1, 1989·Antimicrobial Agents and Chemotherapy·C C HallN H Georgopapadakou
Aug 27, 1990·Biochimica Et Biophysica Acta·J P AbrahamsB Kraal

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