A mutant in the CsDET2 gene leads to a systemic brassinosteriod deficiency and super compact phenotype in cucumber (Cucumis sativus L.)

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Shanshan HouZheng Li

Abstract

A novel dwarf cucumber mutant, scp-2, displays a typical BR biosynthesis-deficient phenotype, which is due to a mutation in CsDET2 for a steroid 5-alpha-reductase. Brassinosteroids (BRs) are a group of plant hormones that play important roles in the development of plant architecture, and extreme dwarfism is a typical outcome of BR-deficiency. Most cucumber (Cucumis sativus L.) varieties have an indeterminate growth habit, and dwarfism may have its value in manipulation of plant architecture and improve production in certain production systems. In this study, we identified a spontaneous dwarf mutant, super compact-2 (scp-2), that also has dark green, wrinkle leaves. Genetic analyses indicated that scp-2 was different from two previously reported dwarf mutants: compact (cp) and super compact-1 (scp-1). Map-based cloning revealed that the mutant phenotype was due to two single nucleotide polymorphism and a single-base insertion in the CsDET2 gene that resulted in a missense mutation in a conserved amino acid and thus a truncated protein lacking the conserved catalytic domains in the predicted steroid 5α-reductase protein. Measurement of endogenous hormone levels indicated a reduced level of brassinolide (BL, a bioactive BR) in scp...Continue Reading

References

Jan 1, 1994·Annual Review of Biochemistry·D W Russell, J D Wilson
Feb 13, 2001·Plant Physiology·C M Steber, P McCourt
Jun 13, 2002·Physiologia Plantarum·Gregory M SymonsJames B Reid
Dec 21, 2002·Trends in Genetics : TIG·Peter Hedden
Jun 27, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·G FazioM R Stevens
Nov 18, 2003·The Plant Journal : for Cell and Molecular Biology·Yoshihito SuzukiIsomaro Yamaguchi
Dec 17, 2003·Journal of Plant Growth Regulation·Gerard J. Bishop
Feb 14, 2004·Plant Physiology·David AlabadíJosé L García-Martínez
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Steven D. Clouse, Jenneth M. Sasse
Jun 3, 2008·Journal of Experimental Botany·Feng Qing FuJing Quan Yu
Aug 21, 2008·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Zheng LiRun Cai
Nov 5, 2008·The Plant Journal : for Cell and Molecular Biology·Li WangKang Chong
Mar 17, 2010·Proceedings of the National Academy of Sciences of the United States of America·Qianqian YeXuelu Wang
May 14, 2011·The New Phytologist·Xiao-Jian XiaJing-Quan Yu
Jul 8, 2011·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Yuhong LiYiqun Weng
Aug 2, 2011·Current Opinion in Plant Biology·Gustavo E Gudesblat, Eugenia Russinova
Nov 23, 2011·Proceedings of the National Academy of Sciences of the United States of America·Thomas HartwigBurkhard Schulz
Apr 11, 2012·The Plant Journal : for Cell and Molecular Biology·Luming YangYiqun Weng
Sep 10, 2015·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Junyi TanZheng Li
Apr 20, 2016·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Tao LinSanwen Huang

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Citations

Nov 23, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Shengjun FengHuasen Wang
Oct 17, 2018·International Journal of Molecular Sciences·Chenggang XiangChanglin Wang
Jan 8, 2020·International Journal of Molecular Sciences·Haileslassie GebremeskelWenge Liu
Dec 20, 2019·Journal of Experimental Botany·Tegan Armarego-MarriottŁucja Kowalewska
Nov 3, 2020·Frontiers in Plant Science·Zhuoyun Wei, Jia Li
Jul 2, 2021·Horticulture Research·Xiaofeng LiuXiaolan Zhang
Jul 9, 2021·Environmental Science and Pollution Research International·Farwa BasitYajing Guan
Aug 15, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Gangjun ZhaoHaibin Wu
Nov 30, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Shuai LiZhonghua Zhang

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Datasets Mentioned

BETA
SSR16667

Methods Mentioned

BETA
PCR
transgenic
genotyping

Software Mentioned

DNAMAN
ClustalW
MEGA

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