A mutant O-GlcNAcase as a probe to reveal global dynamics of protein O-GlcNAcylation during Drosophila embryonic development

The Biochemical Journal
Daniel MariappaDaan M F vanAalten

Abstract

O-GlcNAcylation is a reversible type of serine/threonine glycosylation on nucleocytoplasmic proteins in metazoa. Various genetic approaches in several animal models have revealed that O-GlcNAcylation is essential for embryogenesis. However, the dynamic changes in global O-GlcNAcylation and the underlying mechanistic biology linking them to embryonic development is not understood. One of the limiting factors towards characterizing changes in O-GlcNAcylation has been the limited specificity of currently available tools to detect this modification. In the present study, harnessing the unusual properties of an O-GlcNAcase (OGA) mutant that binds O-GlcNAc (O-N-acetylglucosamine) sites with nanomolar affinity, we uncover changes in protein O-GlcNAcylation as a function of Drosophila development.

References

May 1, 1987·The Journal of Cell Biology·C M SnowL Gerace
May 10, 2000·Proceedings of the National Academy of Sciences of the United States of America·R ShafiJ D Marth
Dec 25, 2003·Journal of the American Chemical Society·Nelly KhidekelLinda C Hsieh-Wilson
May 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Aug 25, 2004·Analytical Biochemistry·Zaneta Nikolovska-ColeskaShaomeng Wang
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Mar 8, 2005·Proteomics·Jana Alonso, Juan F Santarén
Mar 17, 2006·The EMBO Journal·Francesco V RaoDaan M F van Aalten
Aug 2, 2006·The Biochemical Journal·Sarah H ConnerDaan M F Van Aalten
Dec 21, 2006·Journal of the American Chemical Society·Helge C DorfmuellerDaan M F van Aalten
May 15, 2007·Nature Chemical Biology·Nelly KhidekelLinda C Hsieh-Wilson
Jun 4, 2008·The Journal of Biological Chemistry·Stephen A WhelanGerald W Hart
Jul 18, 2008·Glycoconjugate Journal·Vanessa DehennautJean-François Bodart
Aug 8, 2008·Journal of the American Chemical Society·Peter M ClarkLinda C Hsieh-Wilson
Mar 12, 2009·The Biochemical Journal·Helge C DorfmuellerDaan M F van Aalten
Apr 23, 2009·BMC Developmental Biology·Danielle M WebsterScott T Dougan
May 30, 2009·Science·Maria Cristina GambettaJürg Müller
Feb 8, 2011·Cell·Francesca CapotostiWinship Herr
Feb 7, 2012·The EMBO Journal·Shalini PathakDaan Mf van Aalten
Mar 1, 2012·Chemistry & Biology·Marianne SchimplDaan M F van Aalten
Mar 1, 2012·Science Signaling·Daniel MariappaH-Arno J Müller
Jul 10, 2012·Transcription·Zaffer Zargar, Shweta Tyagi
Mar 26, 2013·Biochemical and Biophysical Research Communications·Mitsutaka OgawaKazuo Kamemura
Feb 28, 2014·The Journal of Biological Chemistry·Yuko Tashima, Pamela Stanley
Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Pablo T RadermacherChristian F Lehner
May 7, 2014·Glycobiology·Zhengliang L WuJoseph Zaia
Oct 23, 2014·The Journal of Biological Chemistry·Zhiyuan Ma, Keith Vosseller
Oct 23, 2014·The Journal of Biological Chemistry·Yanping ZhuDavid J Vocadlo
Dec 4, 2014·Developmental Cell·Maria Cristina Gambetta, Jürg Müller

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Citations

Jan 6, 2017·ACS Chemical Biology·Matthew WorthJiaoyang Jiang
Jun 13, 2017·Nature Chemical Biology·Nithya SelvanDaan M F van Aalten
Jun 7, 2017·The Journal of Biological Chemistry·Anke P WillemsDirk J Lefeber
Mar 29, 2018·The Journal of Biological Chemistry·Daniel MariappaDaan M F van Aalten
Sep 11, 2018·Chembiochem : a European Journal of Chemical Biology·Chia-Wei HuJiaoyang Jiang
Feb 26, 2020·The Journal of Biological Chemistry·Villo MuhaDaan M F van Aalten
Mar 25, 2020·Chemistry : a European Journal·Arielis EstevezJiaoyang Jiang
Feb 23, 2020·European Journal of Human Genetics : EJHG·Veronica M PravataDaan M F van Aalten
Nov 15, 2015·The Biochemical Journal·Robert J Chalkley
Apr 9, 2019·Open Biology·Feifei ZhuKeping Chen
Jan 16, 2019·Acta Crystallographica. Section D, Structural Biology·Alexandra Males, Gideon J Davies
Jan 14, 2021·Biochemical Society Transactions·Anastasia ChernykhMorten Thaysen-Andersen
Sep 18, 2020·Molecular & Cellular Proteomics : MCP·Nicholas M RileySharon J Pitteri
Sep 18, 2020·Molecular & Cellular Proteomics : MCP·Jason Maynard, Robert J Chalkley
Oct 27, 2020·The Analyst·Chuan Fu, Daan M F van Aalten
Feb 22, 2021·The Journal of Biological Chemistry·Villő MuhaDaan M F van Aalten
Jul 31, 2020·Physiological Reviews·John C ChathamAdam R Wende
Feb 16, 2021·Molecular & Cellular Proteomics : MCP·Nicholas M RileySharon J Pitteri
Feb 16, 2021·Molecular & Cellular Proteomics : MCP·Jason C Maynard, Robert J Chalkley
Jun 9, 2021·Chembiochem : a European Journal of Chemical Biology·Eun Ju Kim
Aug 3, 2021·Chemical Society Reviews·Abhijit SahaAlberto Fernández-Tejada

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