PMID: 4895215Mar 1, 1969Paper

A mutant of Escherichia coli permitting replication of two F factors

Proceedings of the National Academy of Sciences of the United States of America
W K Maas, A D Goldschmidt

Abstract

Control mechanisms of replication of bacterial genetic elements are poorly understood at present. We have studied one such mechanism involving replication of the F factor in Escherichia coli. The F factor can replicate either autonomously in F(+) or F' strains, or as an integral part of the chromosome in Hfr strains. We have shown that presence of either an integrated F factor or a free F factor prevents replication of a second free F factor. Two integrated F factors can replicate in the same cell. The present experiments show that when a F'lac element was transferred by mating into an Hfr strain, it had to become integrated into the chromosome in order to persist. With a recombination-deficient (recA) Hfr strain as recipient, the frequency of F'lac integration was greatly reduced. This permitted us to isolate a mutant Hfr strain in which the F'lac element was able to replicate autonomously. The mutation has most likely occurred in the integrated F factor itself. Availability of this new recA mutant Hfr strain facilitates genetic analysis of the F factor, since in recA(+) Hfr strains frequent integration of a free F factor into the chromosome obscures recognition of complementation and recombination between two free F factors.

References

May 1, 1968·Proceedings of the National Academy of Sciences of the United States of America·B Low
Dec 1, 1963·Proceedings of the National Academy of Sciences of the United States of America·R MAAS
Feb 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·A J CLARK, A D MARGULIES
May 11, 1962·Biochemical and Biophysical Research Communications·J SCAIFE, J D GROSS

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Citations

Sep 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A J Clark
Jan 1, 1984·Molecular & General Genetics : MGG·S PalchaudhuriJ Murthy
Jan 18, 1977·Molecular & General Genetics : MGG·A F Jamieson, P L Bergquist
Apr 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·R PressW K Maas
Jul 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·S R PalchaudhuriA K Kleinschmidt
Jan 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S Hiraga
May 21, 1974·Molecular & General Genetics : MGG·F San BlasP Broda
Dec 1, 1972·Bacteriological Reviews·K B Low
Jul 1, 1973·Journal of Bacteriology·J K DeVries, W K Maas
Nov 1, 1972·Journal of Bacteriology·Y A ChabbertD H Bouanchaud
Dec 1, 1975·Journal of Bacteriology·C Monti-Bragadin, L Samer

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