PMID: 6165879Jan 1, 1981Paper

A mutant rho ATPase from Escherichia coli that is temperature-sensitive in the presence of RNA

Molecular & General Genetics : MGG
R B Kent, S K Guterman

Abstract

The Escherichia coli mutant rho-115 suppresses lac operon polarity conferred by the lacZ::IS1 insertion MS319. The ATPase activity of purified rho-115 protein was maximal at 40 degrees C, in contrast to 45 degrees C for rho+. At higher temperatures (50 degrees C, 55 degrees C), the fractions of activities at maximal temperature were consistently lower for rho-115 compared to rho+. The 30-minute time course of rho-115 ATP hydrolysis was linear at 37 degrees C but at 45 degrees C the linear kinetics of hydrolysis reached a plateau between 10 and 15 minutes. The 30-minute time courses for rho+ were linear at both 37 degrees C and 45 degrees C. The rho-115 and rho+ ATPase activities were equally heat-stable during preincubation at 45 degrees C in buffer. Inclusion of ATP during preincubation protected these rho proteins from inactivation to the same extent. The presence of polyC during preincubation protected rho+ activity but produced substantial inactivation of rho-115 ATPase. The presence of polyU during preincubation gave similar results. Concentrations of polyC between 625 ng/ml and 100 micrograms/ml yielded the same extent of rho-115 ATPase inactivation during preincubation at 45 degrees C. Thermal inactivation of rho-115 ATP...Continue Reading

References

Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·A DasS Adhya
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·A DasS Adhya
May 25, 1977·Journal of Molecular Biology·L P GuarenteJ Beckwith
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F Lee, C Yanofsky
Jan 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·L P Guarente, J Beckwith
Jan 16, 1979·Molecular & General Genetics : MGG·S K Guterman, C L Howitt
Apr 5, 1979·Journal of Molecular Biology·L Guarente
May 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J P RichardsonC Lowery
Dec 1, 1975·Journal of Bacteriology·S K GutermanD H Boyd
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·C Lowery-Goldhammer, J P Richardson
Jun 15, 1973·European Journal of Biochemistry·J L Darlix
Aug 15, 1973·Journal of Molecular Biology·E G Minkley
Jan 1, 1966·Cold Spring Harbor Symposia on Quantitative Biology·M H Malamy
Apr 15, 1980·Journal of Molecular Biology·G R Galluppi, J P Richardson
Nov 1, 1964·Analytical Biochemistry·T JOVINM A NAUGHTON

❮ Previous
Next ❯

Citations

Jan 1, 1984·Molecular & General Genetics : MGG·H KungD H Calhoun
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R B Kent, S K Guterman
Jan 15, 1985·European Journal of Biochemistry·D O Nwankwo, S K Guterman
Jun 1, 1982·Journal of Bacteriology·S K GutermanB Tyler

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.