PMID: 9657334Jul 10, 1998Paper

A mutation scanning approach for the identification of hookworm species and analysis of population variation

Molecular and Biochemical Parasitology
R B GasserN B Chilton

Abstract

To overcome limitations in the morphological identification of different developmental stages of hookworms to species, we have established a polymerase chain reaction-linked single strand conformation polymorphism technique (PCR-SSCP) utilizing the internal transcribed spacers (ITS) of ribosomal (r)DNA. These spacers were specifically chosen because they provide reliable species markers for strongylid nematodes. ITS spacers were amplified by PCR from DNA derived from individual parasites of seven species of hookworm, then denatured and subjected to electrophoresis in a mutation detection enhancement (MDE) (non-denaturing) gel matrix. PCR SSCP analysis showed that the single-strand ITS patterns produced allowed the unequivocal identification of all species. The method also allowed the direct display of sequence variation within some species where multiple individual worms were examined. These findings demonstrate the usefulness of the SSCP approach for hookworm identification, the detection of population variation and the direct display of sequence variation in rDNA.

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Citations

Dec 20, 2008·Parasitology Research·Josefine CarlsgartPeter Nejsum
May 12, 2004·Molecular and Cellular Probes·Marion JohnsonRobin B Gasser
Mar 8, 2000·International Journal for Parasitology·L van HerwerdenD Blair
Mar 25, 2000·International Journal for Parasitology·R B Gasser, S E Newton
Jan 30, 1999·International Journal for Parasitology·X ZhuN B Chilton
May 20, 1999·International Journal for Parasitology·X ZhuR B Gasser
Mar 8, 2000·International Journal for Parasitology·J J VerweijR B Gasser
Mar 25, 2000·International Journal for Parasitology·M Blaxter
Aug 24, 1999·Veterinary Parasitology·R B Gasser
Nov 3, 2011·PloS One·Yue XieGuangyou Yang
Dec 1, 1999·International Journal for Parasitology·R B Gasser, P O'Donoghue
Jun 9, 2005·Tropical Medicine & International Health : TM & IH·Johanna M de GruijterAnton M Polderman
May 24, 2006·Molecular and Cellular Probes·Johanna M de GruijterRobin B Gasser
Sep 14, 2006·The Journal of Eukaryotic Microbiology·Christopher M Whipps, Michael L Kent
Dec 23, 2008·Expert Review of Molecular Diagnostics·Robin B GasserBronwyn E Campbell
Feb 22, 2017·Journal of Helminthology·X L ShiG Q Li
Dec 7, 2018·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Kerla Joeline Lima MonteiroFilipe Anibal Carvalho-Costa
Jun 18, 2021·Parasitology International·Khanh-Linh BuiAyako Yoshida
Jul 25, 2007·Journal of Agricultural and Food Chemistry·Maria Jose ChapelaCarmen G Sotelo

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