A myosin II nanomachine mimicking the striated muscle

Nature Communications
Irene PerticiPasquale Bianco

Abstract

The contraction of striated muscle (skeletal and cardiac muscle) is generated by ATP-dependent interactions between the molecular motor myosin II and the actin filament. The myosin motors are mechanically coupled along the thick filament in a geometry not achievable by single-molecule experiments. Here we show that a synthetic one-dimensional nanomachine, comprising fewer than ten myosin II dimers purified from rabbit psoas, performs isometric and isotonic contractions at 2 mM ATP, delivering a maximum power of 5 aW. The results are explained with a kinetic model fitted to the performance of mammalian skeletal muscle, showing that the condition for the motor coordination that maximises the efficiency in striated muscle is a minimum of 32 myosin heads sharing a common mechanical ground. The nanomachine offers a powerful tool for investigating muscle contractile-protein physiology, pathology and pharmacology without the potentially disturbing effects of the cytoskeletal-and regulatory-protein environment.

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Citations

Mar 26, 2019·Small·Fei WangQian Li
Feb 23, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alberto Ciferri, Alvin L Crumbliss
Oct 17, 2019·American Journal of Physiology. Cell Physiology·Yu-Shu ChengDilson E Rassier
Jul 19, 2020·Biomechanics and Modeling in Mechanobiology·François Kimmig, Matthieu Caruel
Oct 11, 2020·International Journal of Molecular Sciences·Irene PerticiPasquale Bianco
Jan 23, 2020·Biophysical Journal·Marco LinariVincenzo Lombardi
Jun 25, 2021·Frontiers in Microbiology·Soichiro KijimaHiroyuki Ogata

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Methods Mentioned

BETA
optical
motility assay
Assay
light scattering
electrophoresis
force measurement

Software Mentioned

Origin
LabVIEW

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