A nano-view of West Nile virus-induced cellular changes during infection

Journal of Nanobiotechnology
Jason WM Lee, Mah Lee Ng

Abstract

BACKGROUND: Microscopic imaging of viruses and their interactions with and effects on host cells are frequently held back by limitations of the microscope's resolution or the invasive nature of the sample preparation procedures. It is also difficult to have a technique that would allow simultaneous imaging of both surface and sub-surface on the same cell. This has hampered endeavours to elucidate virus-host interactions. Atomic Force Microscopy (AFM), which is commonly used in the physical sciences, is now becoming a good correlative form of microscopy used to complement existing optical, confocal and electron microscopy for biological applications RESULTS: In this study, the West Nile (Sarafend) virus-infected Vero cell model was used. The atomic force microscope was found to be useful in producing high resolution images of virus-host events with minimal sample processing requirements. The AFM was able to image the budding of the West Nile (Sarafend) virus at the infected cell surface. Proliferation of the filopodia and thickening of clusters of actin filaments accompanied West Nile virus replication. CONCLUSIONS: The study shows that the AFM is useful for virus-host interaction studies. The technique provides morphological in...Continue Reading

References

Jan 1, 1985·Intervirology·E G WestawayD W Trent
Jan 1, 1994·Archives of Virology·M L NgJ J Mulders
Jan 1, 1994·Micron : the International Research and Review Journal for Microscopy·N AlmqvistS Fredriksson
Oct 23, 1997·Biophysical Journal·F M OhnesorgeG Binnig
Apr 29, 1998·FEBS Letters·Y F DryginI V Yaminsky
Sep 11, 2003·Journal of Medical Virology·J J H ChuM L Ng
Mar 17, 2004·Biomaterials·Meng Yu, Albena Ivanisevic

❮ Previous
Next ❯

Citations

Sep 29, 2005·Journal of Nanobiotechnology·Sunil KumarSujoy K Guha
Dec 19, 2007·Biochimica Et Biophysica Acta·Tuan Lin TanWei Ning Chen
Feb 7, 2019·Virologica Sinica·Yue ZhangYaming Jiu
Jan 9, 2016·The Science of the Total Environment·Yan WangBernd Nowack

❮ Previous
Next ❯

Methods Mentioned

BETA
AFM
atomic force microscopy
FCS

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Micron : the International Research and Review Journal for Microscopy
Mun Keat ChongMah Lee Ng
Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi
Yunfeng Shi, Jiye Cai
Forensic Science International : Synergy
S KasasG Dietler
© 2022 Meta ULC. All rights reserved