A near-infrared fluorescent probe with large Stokes shift for imaging Cys in tumor mice.

Analytica Chimica Acta
Juan LiuChun-Yan Li

Abstract

Cysteine (Cys), a kind of small molecule biological thiol, not only involves in the regulation of physiological processes, but also is considered a marker of tumor. However, it is challenging to develop suitable probe for detecting Cys in tumors. In this paper, a near-infrared (NIR) fluorescent probe named IX for Cys has been designed and synthesized. The probe shows a NIR emission peak at 770 nm with large Stokes shift (180 nm) upon adding Cys. It displays high sensitivity to Cys with 6-fold increase of fluorescence intensity. Meanwhile, IX has the high selectivity to Cys over other potential interference such as Hcy and GSH, which have similar structure with Cys. In addition, a possible mechanism of fluorescence enhancement is the reaction of IX with Cys to release IX-OH, which is verified by fluorescence spectra, MS and HPLC. Next, IX can selectively image Cys in HCT-116 cells thanks to the low cytotoxicity. Most important of all, the fluorescent probe IX has visualized Cys in HCT116-xenograft tumor mice due to the near-infrared properties with large Stokes shift.

References

Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·M W LiebermanM M Matzuk
Jan 9, 2003·Trends in Biochemical Sciences·Zachary A WoodLeslie B Poole
Nov 19, 2010·Nature·Eranthie WeerapanaBenjamin F Cravatt
May 24, 2012·Chemical Reviews·Marc VendrellYoung-Tae Chang
May 22, 2013·Chemical Society Reviews·Hyo Sung JungJuyoung Yoon
Jan 28, 2015·Chemical Society Reviews·Matteo StaderiniJ Carlos Menéndez
Feb 9, 2017·Journal of the American Chemical Society·Yongkang YueCaixia Yin
Aug 29, 2017·Journal of the American Chemical Society·Ningning WangJishan Li
Mar 14, 2018·Trends in Pharmacological Sciences·Bindu D PaulSolomon H Snyder
Apr 25, 2018·Analytical Chemistry·Chunyang ChenWeisheng Liu
Apr 9, 2019·Chemical Communications : Chem Comm·Erman KarakuşMustafa Emrullahoğlu
Apr 16, 2019·Molecular Biology and Evolution·Damián Alvarez-PaggiLeonardo G Alonso
Jun 19, 2019·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Miao LiChuan Dong
Apr 4, 2020·Analytical Chemistry·Yongkang YueCaixia Yin
Jul 2, 2020·Analytical Chemistry·Lu JiaQing-Zheng Yang
Aug 19, 2020·Current Biology : CB·Nicole M Anderson, M Celeste Simon
Aug 19, 2020·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Kailasam Saravana ManiSubramaniam Parameswaran Rajendran
Nov 7, 2020·Chemical Society Reviews·Xuechen LiWeiying Lin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.