A negative feedback loop involving small RNAs accelerates Vibrio cholerae's transition out of quorum-sensing mode

Genes & Development
Sine L SvenningsenB L Bassler

Abstract

Quorum sensing is a cell-to-cell communication process that allows bacteria to measure their population numbers and to synchronously alter gene expression in response to changes in cell population density. At the core of the Vibrio cholerae quorum-sensing signal transduction pathway lie four redundant small RNAs (sRNAs), named the Quorum Regulatory RNAs (Qrr1-4). Expression of qrr1-4 is cell population density-dependent due to a requirement for the quorum-sensing controlled phosphorylated response regulator LuxO-P, which is abundant only at low cell population density. When expressed, Qrr1-4 repress translation of HapR, the "master" quorum-sensing transcription factor. Here we show a negative feedback loop in which HapR activates transcription of the qrr genes, which indirectly leads to hapR repression. Efficient feedback activation of the qrr genes requires the simultaneous presence of LuxO-P (present only at low cell population density) and HapR (present only at high cell population density). For this reason, the feedback loop does not influence quorum sensing at steady-state low or high cell population density. However, LuxO-P and HapR are simultaneously present immediately following the switch from high to low cell density ...Continue Reading

References

Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J W RobertsN L Craig
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J W Little
May 1, 1982·Journal of Bacteriology·C F BeckW Müller
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J Engebrecht, M Silverman
Feb 1, 1994·Molecular Microbiology·W Pan, B G Spratt
Feb 22, 1996·Gene·K Skorupski, R K Taylor
Apr 1, 1996·Molecular Microbiology·J ChatterjeeE A Meighen
Jul 14, 2000·Journal of Bacteriology·M BuckJ D Gralla
Jun 16, 2001·Advances in Microbial Physiology·J G Coote
Nov 6, 2002·Journal of Molecular Biology·Nitzan RosenfeldUri Alon
Mar 5, 2003·Current Opinion in Microbiology·Roger S Smith, Barbara H Iglewski
May 7, 2003·Current Opinion in Microbiology·Karina B Xavier, Bonnie L Bassler
Sep 17, 2003·Genes & Development·Eric MasséSusan Gottesman
Jun 1, 1961·Journal of Molecular Biology·F JACOB, J MONOD
Sep 26, 2003·Molecular Microbiology·Brian K Hammer, Bonnie L Bassler
Oct 11, 2003·Developmental Cell·Jun Zhu, John J Mekalanos
Apr 23, 2004·Current Opinion in Structural Biology·Kerren K Swinger, Phoebe A Rice
Jun 24, 2004·Infection and Immunity·H H KrishnanRukhsana Chowdhury
Nov 3, 2004·Trends in Cell Biology·Jennifer M Henke, Bonnie L Bassler
Apr 2, 2005·Current Opinion in Microbiology·John S ParkinsonClaudia A Studdert
Oct 11, 2005·Annual Review of Cell and Developmental Biology·Christopher M Waters, Bonnie L Bassler
Nov 3, 2005·Molecular Microbiology·Derrick H LenzBonnie L Bassler
Nov 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Elisabeth KayDieter Haas
Feb 17, 2006·Current Opinion in Microbiology·Dagmar Beier, Roy Gross
Mar 24, 2006·Nature·Gürol M SüelMichael B Elowitz

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Citations

Mar 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Wai-Leung NgBonnie L Bassler
Oct 29, 2010·Cold Spring Harbor Perspectives in Biology·Susan Gottesman, Gisela Storz
Feb 18, 2011·Genes & Development·Steven T RutherfordBonnie L Bassler
Oct 27, 2009·Journal of Bacteriology·Ho-Ching Tiffany TsuiMalcolm E Winkler
Aug 19, 2009·Annual Review of Genetics·Wai-Leung Ng, Bonnie L Bassler
Mar 3, 2010·Annual Review of Biophysics·Kim SneppenSzabolcs Semsey
Jan 9, 2013·Journal of Molecular Microbiology and Biotechnology·Rafi AhmadPeik Haugen
Sep 11, 2013·Journal of Theoretical Biology·Geoffrey A M Hunter, James P Keener
Jul 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Zhi LiuJun Zhu
Feb 3, 2016·Nature Communications·Michal Brunwasser-MeiromRoee Amit
Jan 24, 2009·The EMBO Journal·Sine L SvenningsenBonnie L Bassler
Apr 7, 2016·Journal of Receptor and Signal Transduction Research·Sundaraj Rajamanikandan, Pappu Srinivasan
Jul 29, 2010·FEMS Microbiology Reviews·Chase L Beisel, Gisela Storz
May 2, 2012·RNA Biology·J Patrick Bardill, Brian K Hammer
Nov 22, 2013·Gut Microbes·Francesca P Rothenbacher, Jun Zhu
Mar 26, 2015·Indian Journal of Microbiology·M HemaS Adline Princy
Nov 13, 2014·Frontiers in Cellular and Infection Microbiology·Clayton C CaswellErin R Murphy
Apr 12, 2014·Frontiers in Cellular and Infection Microbiology·Luary C Martínez, Viveka Vadyvaloo
Aug 12, 2016·Nature Reviews. Microbiology·Kai Papenfort, Bonnie L Bassler
Aug 17, 2016·ACS Synthetic Biology·Maciej B HolowkoChueh Loo Poh
Feb 14, 2015·Current Opinion in Microbiology·Sebastian M Castillo-HairJeffrey J Tabor
Feb 24, 2017·International Journal of Medical Microbiology : IJMM·Philipp WurmJoachim Reidl
May 10, 2017·Journal of Bacteriology·Alyssa S BallJulia C van Kessel
Oct 15, 2014·Journal of Bacteriology·Julia C van KesselBonnie L Bassler
Sep 24, 2008·Molecular Microbiology·Kimberly C TuBonnie L Bassler
Apr 20, 2010·Molecular Microbiology·Christophe BordiAlain Filloux
Jan 19, 2012·Molecular Microbiology·Mikkel Girke JørgensenPoul Valentin-Hansen

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