PMID: 9636211Jun 24, 1998Paper

A negative regulator mediates quorum-sensing control of exopolysaccharide production in Pantoea stewartii subsp. stewartii

Proceedings of the National Academy of Sciences of the United States of America
S B von BodmanD L Coplin

Abstract

Classical quorum-sensing (autoinduction) regulation, as exemplified by the lux system of Vibrio fischeri, requires N-acyl homoserine lactone (AHL) signals to stimulate cognate transcriptional activators for the cell density-dependent expression of specific target gene systems. For Pantoea stewartii subsp. stewartii, a bacterial pathogen of sweet corn and maize, the extracellular polysaccharide (EPS) stewartan is a major virulence factor, and its production is controlled by quorum sensing in a population density-dependent manner. Two genes, esaI and esaR, encode essential regulatory proteins for quorum sensing. EsaI is the AHL signal synthase, and EsaR is the cognate gene regulator. esaI, DeltaesaR, and DeltaesaI-esaR mutations were constructed to establish the regulatory role of EsaR. We report here that strains containing an esaR mutation produce high levels of EPS independently of cell density and in the absence of the AHL signal. Our data indicate that quorum-sensing regulation in P. s. subsp. stewartii, in contrast to most other described systems, uses EsaR to repress EPS synthesis at low cell density, and that derepression requires micromolar amounts of AHL. In addition, derepressed esaR strains, which synthesize EPS const...Continue Reading

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Citations

Sep 6, 2006·Analytical and Bioanalytical Chemistry·William Nasser, Sylvie Reverchon
Oct 5, 2006·Analytical and Bioanalytical Chemistry·Diane McDougaldStaffan Kjelleberg
May 24, 2005·Extremophiles : Life Under Extreme Conditions·Inmaculada LlamasJuan E González
Apr 20, 2006·Antonie van Leeuwenhoek·Inmaculada LlamasEmilia Quesada
Jun 18, 2003·International Journal of Food Microbiology·S K Anand, Mansel W Griffiths
Aug 29, 2001·FEMS Microbiology Reviews·N A WhiteheadG P Salmond
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Dec 4, 2010·Chemical Reviews·Mair E A Churchill, Lingling Chen
Jun 16, 2004·Nature Reviews. Microbiology·Andrée M LazdunskiJames N Sturgis
Jul 27, 2005·Molecular Plant-microbe Interactions : MPMI·Beatriz QuiñonesSteven E Lindow
Sep 13, 2008·Molecular Plant-microbe Interactions : MPMI·Carmen M HerreraSusanne B von Bodman
May 15, 2004·Molecular Plant-microbe Interactions : MPMI·Beatriz QuiñonesSteven E Lindow
Mar 16, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Anne M L BarnardGeorge P C Salmond
Jul 24, 2012·Applied and Environmental Microbiology·Mojtaba MohammadiM Caroline Roper
Apr 7, 2004·Applied and Environmental Microbiology·Dacheng RenThomas K Wood
Oct 6, 2005·Applied and Environmental Microbiology·Lynn L WilliamsonJo Handelsman
Mar 5, 2005·Applied and Environmental Microbiology·Ya-Juan Wang, Jared Renton Leadbetter
Jul 8, 2005·Applied and Environmental Microbiology·Dacheng RenThomas K Wood
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Dec 18, 2002·Journal of Bacteriology·Melanie M MarketonJuan E González
Jul 3, 2004·Journal of Bacteriology·Matthew R Parsek, Clay Fuqua

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