PMID: 9420617Jan 8, 1998Paper

A neoepitope-based enzyme immunoassay for quantification of C1-inhibitor in complex with C1r and C1s

Scandinavian Journal of Immunology
H FureTom E Mollnes

Abstract

Monoclonal antibodies (MoAb) recognizing neoepitopes exposed on activation products of complement proteins but hidden in the native components have been used for quantification of activated complement. A previously produced and characterized mouse MoAb, recognizing a neoepitope on the human plasma protein C1-inhibitor complexed with its substrates, was used to design an enzyme immunoassay for detection of C1-inhibitor complexed with C1r and C1s. These complexes are indicators of early classical complement pathway activation. The standard was serum activated with heat aggregated IgG defined to contain 1000 arbitrary units (AU)/ml. The lower detection limit was approximately 0.05 AU/ml corresponding to 0.005% of fully activated serum. The reliability of the assay, including day-to-day variation, was tested. Intra-assay variation coefficients were 12% for low plasma control and 13% for high plasma control (n = 12 for both). Inter-assay variation coefficients were 12% for low control (n = 6), 19% for high control (n = 6) and 15% for the normal plasma control (n = 9). A 2.5-97.5 percentile reference range (normal blood donors) was 16-33 AU/ml. Two patients with systemic lupus erythematosus had considerably elevated plasma levels of ...Continue Reading

Citations

May 8, 2007·Transactions of the Royal Society of Tropical Medicine and Hygiene·Meta RoestenbergAndré van der Ven
Mar 10, 2001·Obstetrics and Gynecology·J R MellembakkenV Videm
Jul 4, 1998·Scandinavian Journal of Immunology·A E FianeT E Mollnes
Sep 22, 1998·Scandinavian Journal of Immunology·T E MollnesR Perricone
Jul 6, 2001·Scandinavian Journal of Immunology·E UlvestadT E Mollnes
Feb 19, 1999·AIDS Research and Human Retroviruses·A BerettaA Gringeri
Dec 12, 2003·BMC Physiology·Bjørn Audun RisøyHaakon B Benestad
Nov 8, 2003·Pediatric Research·Albert CastellheimTom E Mollnes
Mar 1, 2012·Acta Biomaterialia·Anders HovlandKnut Tore Lappegård
Jun 15, 2011·Advanced Drug Delivery Reviews·Morten HarboeTom Eirik Mollnes
Sep 5, 2007·Molecular Immunology·Tom Eirik MollnesMichael Kirschfink
Jul 9, 2004·Scandinavian Journal of Immunology·K T LappegårdT E Mollnes
Aug 4, 2006·Pacing and Clinical Electrophysiology : PACE·Knut T Lappegård, Hanne Bjørnstad
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Feb 23, 2010·The Annals of Thoracic Surgery·Cornelia HoedemaekersJohannes van der Hoeven
Mar 3, 2004·The Annals of Thoracic Surgery·Knut Tore LappegårdTom Eirik Mollnes
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