A nerve growth factor-induced gene encodes a possible transcriptional regulatory factor

J Milbrandt


Nerve growth factor (NGF) is a trophic agent that promotes the outgrowth of nerve fibers from sympathetic and sensory ganglia. The neuronal differentiation stimulated by this hormone was examined in the NGF-responsive cell line PC12. Differential hybridization was used to screen a complementary DNA library constructed from PC12 cells treated with NGF and cycloheximide. One of the complementary DNA clones that was rapidly induced by NGF was found to have a nucleotide sequence that predicts a 54-kilodalton protein with homology to transcriptional regulatory proteins. This clone, NGFI-A, contains three tandemly repeated copies of the 28- to 30-amino acid "zinc finger" domain present in Xenopus laevis TFIIIA and other DNA-binding proteins. It also contains another highly conserved unit of eight amino acids that is repeated at least 11 times. The NGFI-A gene is expressed at relatively high levels in the brain, lung, and superior cervical ganglion of the adult rat.


Apr 29, 1998·The Journal of Comparative Neurology·C V Mello, Sidarta Ribeiro
Jul 1, 1996·Journal of Neuroscience Research·R NäkkiJ Honkaniemi
Nov 2, 2004·Journal of Cellular Biochemistry·Hirohiko OkamuraTatsuji Haneji
Nov 4, 2004·Journal of Cellular Biochemistry·Alia Al-SarrajGerald Thiel
Dec 1, 1994·Journal of Cellular Biochemistry·R P HuangE D Adamson
Apr 1, 1994·Neurochemical Research·D F CondorelliA M Giuffrida-Stella
Jul 1, 1994·Molecular Biology Reports·T Pieler, E Bellefroid
May 1, 1996·Behavior Genetics·J M KornhauserJ S Takahashi
May 20, 1998·Journal of Molecular Neuroscience : MN·A S HazellA M Hakim
Jan 1, 1990·Journal of Molecular Neuroscience : MN·M A SussmanV M Fowler
Jan 1, 1988·Molecular Neurobiology·R E Harlan
Jan 1, 1988·Molecular Neurobiology·A LeviP Calissano
May 3, 2011·Calcified Tissue International·Marie K ReumannPhilipp Mayer-Kuckuk
Sep 29, 2011·Pflügers Archiv : European journal of physiology·Sidarta Ribeiro
Sep 29, 2009·Neuroscience Bulletin·Ming-Gang Liu, Jun Chen
Jan 1, 1994·Neuroscience and Biobehavioral Reviews·L M ManessJ E Zadina
Jul 1, 1995·Brain Research. Brain Research Reviews·M Dragunow, K Preston
Nov 1, 1989·Trends in Neurosciences·J I Morgan, T Curran
Apr 1, 1990·Trends in Neurosciences·U Lendahl, R D McKay
Jul 1, 1990·Brain Research. Molecular Brain Research·K MackD I Gottlieb

Related Concepts

EGR1 wt Allele
Zinc Fingers
EGR1 gene
Nucleic Acid Hybridization Procedure
Amino Acids, I.V. solution additive
Transcription, Genetic
Nerve Growth Factor
PC12 Cells

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.