A nerve growth factor-induced gene encodes a possible transcriptional regulatory factor

Science
J Milbrandt

Abstract

Nerve growth factor (NGF) is a trophic agent that promotes the outgrowth of nerve fibers from sympathetic and sensory ganglia. The neuronal differentiation stimulated by this hormone was examined in the NGF-responsive cell line PC12. Differential hybridization was used to screen a complementary DNA library constructed from PC12 cells treated with NGF and cycloheximide. One of the complementary DNA clones that was rapidly induced by NGF was found to have a nucleotide sequence that predicts a 54-kilodalton protein with homology to transcriptional regulatory proteins. This clone, NGFI-A, contains three tandemly repeated copies of the 28- to 30-amino acid "zinc finger" domain present in Xenopus laevis TFIIIA and other DNA-binding proteins. It also contains another highly conserved unit of eight amino acids that is repeated at least 11 times. The NGFI-A gene is expressed at relatively high levels in the brain, lung, and superior cervical ganglion of the adult rat.

References

Apr 29, 1998·The Journal of Comparative Neurology·C V Mello, Sidarta Ribeiro
Jul 1, 1996·Journal of Neuroscience Research·R NäkkiJ Honkaniemi
Nov 2, 2004·Journal of Cellular Biochemistry·Hirohiko OkamuraTatsuji Haneji
Nov 4, 2004·Journal of Cellular Biochemistry·Alia Al-SarrajGerald Thiel
Dec 1, 1994·Journal of Cellular Biochemistry·R P HuangE D Adamson
Apr 1, 1994·Neurochemical Research·D F CondorelliA M Giuffrida-Stella
Jul 1, 1994·Molecular Biology Reports·T Pieler, E Bellefroid
May 1, 1996·Behavior Genetics·J M KornhauserJ S Takahashi
May 20, 1998·Journal of Molecular Neuroscience : MN·A S HazellA M Hakim
Jan 1, 1990·Journal of Molecular Neuroscience : MN·M A SussmanV M Fowler
Jan 1, 1988·Molecular Neurobiology·R E Harlan
Jan 1, 1988·Molecular Neurobiology·A LeviP Calissano
May 3, 2011·Calcified Tissue International·Marie K ReumannPhilipp Mayer-Kuckuk
Sep 29, 2011·Pflügers Archiv : European journal of physiology·Sidarta Ribeiro
Sep 29, 2009·Neuroscience Bulletin·Ming-Gang Liu, Jun Chen
Jan 1, 1994·Neuroscience and Biobehavioral Reviews·L M ManessJ E Zadina
Jul 1, 1995·Brain Research. Brain Research Reviews·M Dragunow, K Preston
Nov 1, 1989·Trends in Neurosciences·J I Morgan, T Curran
Apr 1, 1990·Trends in Neurosciences·U Lendahl, R D McKay
Jul 1, 1990·Brain Research. Molecular Brain Research·K MackD I Gottlieb

Related Concepts

EGR1 wt Allele
Zinc Fingers
EGR1 gene
Lung
Nucleic Acid Hybridization Procedure
Amino Acids, I.V. solution additive
Transcription, Genetic
Nerve Growth Factor
Brain
PC12 Cells

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