PMID: 3772374Dec 1, 1986Paper

A nerve growth factor-sensitive S6 kinase in cell-free extracts from PC12 cells

Journal of Neurochemistry
Y MatsudaG Guroff

Abstract

Soluble extracts from nerve growth factor (NGF)-stimulated PC12 cells prepared by alkaline lysis show a two- to 10-fold greater ability to phosphorylate the 40S ribosomal protein S6 than do extracts from control cells. The alkaline lysis method yields a preparation of much higher specific activity than does sonication. Half-maximal incorporation of 32P from [32P]ATP into S6 occurred after 4-7 min of NGF treatment. The partially purified NGF-sensitive S6 kinase has a molecular weight of 45,000. It is not inhibited by NaCl, chlorpromazine, or the specific inhibitor of cyclic AMP (cAMP)-dependent protein kinase, nor is it activated by addition of diolein plus phosphatidylserine. Trypsin treatment of either crude extracts or partially purified S6 kinase from control or NGF-treated cells was without effect. These data suggest that the S6 kinase stimulated by NGF is neither cAMP-dependent protein kinase or protein kinase C nor the result of tryptic activation of an inactive proenzyme. Treatment of intact cells with dibutyryl cAMP or 5'-N-ethylcarboxamideadenosine also increases the subsequent cell-free phosphorylation of S6. This observation suggests that cAMP-dependent protein kinase may be involved in the phosphorylation of S6 kinase.

References

Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·E Erikson, J L Maller
Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D TabariniO M Rosen
Jul 1, 1968·Physiological Reviews·R Levi-Montalcini, P U Angeletti
Oct 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·V Bocchini, P U Angeletti
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C J Le PeuchO M Rosen

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Citations

Jan 1, 1988·Molecular Neurobiology·A LeviP Calissano
Jan 1, 1989·Brain Research. Developmental Brain Research·J M GavaretM Pierre
Aug 23, 1993·Biochimica Et Biophysica Acta·K W Wood, T M Roberts
Feb 15, 1988·FEBS Letters·M PierreD Toru-Delbauffe
Aug 27, 1993·Annals of the New York Academy of Sciences·G Guroff
Nov 1, 2016·Journal of Neurochemistry·Steven W Barger

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