A network of stimulatory and inhibitory Galpha-subunits regulates olfaction in Caenorhabditis elegans

Genetics
Hannes LansGert Jansen

Abstract

The two pairs of sensory neurons of C. elegans, AWA and AWC, that mediate odorant attraction, express six Galpha-subunits, suggesting that olfaction is regulated by a complex signaling network. Here, we describe the cellular localization and functions of the six olfactory Galpha-subunits: GPA-2, GPA-3, GPA-5, GPA-6, GPA-13, and ODR-3. All except GPA-6 localize to sensory cilia, suggesting a direct role in sensory transduction. GPA-2, GPA-3, GPA-5, and GPA-6 are also present in cell bodies and axons and GPA-5 specifically localizes to synaptic sites. Analysis of animals with single- to sixfold loss-of-function mutations shows that olfaction involves a balance between multiple stimulatory and inhibitory signals. ODR-3 constitutes the main stimulatory signal and is sufficient for the detection of odorants. GPA-3 forms a second stimulatory signal in the AWA and AWC neurons, also sufficient for odorant detection. In AWA, signaling is suppressed by GPA-5. In AWC, GPA-2 and GPA-13 negatively and positively regulate signaling, respectively. Finally, we show that only ODR-3 plays a role in cilia morphogenesis. Defects in this process are, however, independent of olfactory behavior. Our findings reveal the existence of a complex signalin...Continue Reading

References

May 5, 1990·Journal of Molecular Biology·L van der VoornH L Ploegh
May 1, 1974·Genetics·S Brenner
Jun 15, 1997·Genes & Development·H C KorswagenR H Plasterk
Feb 14, 1998·The Journal of Biological Chemistry·H E Hamm
Feb 12, 1998·Neuron·L BelluscioR Axel
Apr 7, 1999·Nature Genetics·G JansenR H Plasterk
Aug 6, 1999·Brain Research·K S Wekesa, R R Anholt
Sep 15, 2001·Nature·R C Hardie, P Raghu
Sep 15, 2001·Nature·S Firestein
Feb 28, 2002·The EMBO Journal·Gert JansenRonald H A Plasterk
May 29, 2002·Brain Research·Alice H LuoRobert R H Anholt
Aug 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·William M NuttleyDerek Van Der Kooy
Aug 28, 2003·Médecine sciences : M/S·Christelle Gally, Jean-Louis Bessereau
Jul 17, 2008·Comparative and Functional Genomics·Edwin CuppenRonald H A Plasterk
Nov 12, 1986·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J G WhiteS Brenner

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Citations

Jun 21, 2006·Journal of Molecular Evolution·Damien M O'HalloranAnn M Burnell
Jan 9, 2007·Pflügers Archiv : European journal of physiology·Piali Sengupta
Apr 28, 2005·Molecular Genetics and Genomics : MGG·Richard Jovelin, Patrick C Phillips
Mar 15, 2012·Nature Communications·Kazushi YoshidaTakeshi Ishihara
Jun 7, 2011·Nature Neuroscience·Houbin ZhangWolfgang Baehr
Mar 12, 2010·Proceedings of the National Academy of Sciences of the United States of America·Jin I LeeNoelle D L'Etoile
Feb 11, 2009·Genes & Development·Bluma J LeschCornelia I Bargmann
Jul 19, 2005·Annual Review of Neuroscience·Mario de Bono, Andres Villu Maricq
Oct 12, 2012·International Journal of Evolutionary Biology·David A Fitzpatrick, Damien M O'Halloran
Dec 17, 2009·PLoS Genetics·Damien M O'HalloranNoelle D L'Etoile
Jun 19, 2008·PloS One·Christian Frøkjaer-JensenShawn R Lockery
Feb 10, 2012·PloS One·Damien M O'HalloranNoelle D L'Etoile
Feb 26, 2009·Genes & Development·Daniel VasiliauskasClaude Desplan
Aug 19, 2009·Annals of the New York Academy of Sciences·Hannes LansGert Jansen
May 24, 2015·Molecular Phylogenetics and Evolution·Arunkumar KrishnanHelgi B Schiöth
Nov 6, 2013·Biotechnology Advances·Chunmei ZhangXiaowei Huang
May 15, 2008·Developmental Cell·Saikat MukhopadhyayPiali Sengupta
Sep 25, 2012·Vision Research·Ryan ConstantineWolfgang Baehr
May 25, 2010·Behavioural Brain Research·Maria Christina HoffmannDerek van der Kooy
Jun 27, 2017·Frontiers in Physiology·Carla Igual GilAnne-Katrin Rohlfing
Jan 27, 2017·Frontiers in Endocrinology·Ichiro N Maruyama
May 26, 2017·PLoS Genetics·Rosalind HusseySupriya Srinivasan
Jul 20, 2019·Nature Communications·Christopher D ChuteJagan Srinivasan
Mar 12, 2021·Genetics·Denise M FerkeyNoelle D L'Etoile

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