PMID: 2501149May 1, 1989Paper

A neuron-specific enhancer of the Drosophila dopa decarboxylase gene

Genes & Development
W A JohnsonJay Hirsh

Abstract

At least two cis-regulatory elements are necessary for correct neuron-specific expression of the Drosophila melanogaster dopa decarboxylase gene, Ddc. In addition to a previously described proximal element located approximately 60 bp upstream of the mRNA start site, we have now characterized a distal approximately 600-bp DNA fragment, extending from -1019 to -1623 bp, which possesses enhancer-like properties and is essential for normal neuron-specific expression. Immunofluorescent labeling of neurons expressing deleted Ddc genes indicates that this region contains both general neuronal regulatory elements and cell-specific elements that selectively affect Ddc expression in either dopaminergic or serotonergic neurons. These selective effects can be correlated with the removal of sequence elements that are protected from DNase digestion by factors present in embryonic nuclear extracts. Several of these elements are also homologous to sequences located upstream of the evolutionarily diverged Ddc gene of Drosophila virilis. These results suggest that the neuron-specific expression of Ddc results from the combined action of several factors binding within this distal enhancer region.

References

Feb 15, 1988·The Journal of Comparative Neurology·V Budnik, K White
Jul 1, 1987·Developmental Biology·K D Konrad, J L Marsh
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S Tabor, C C Richardson
Dec 1, 1985·Developmental Biology·A Furst, A P Mahowald
Jul 1, 1969·Archives of Biochemistry and Biophysics·K D Lunan, H K Mitchell
Oct 22, 1982·Science·G M Rubin, A C Spradling

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Citations

Aug 1, 1991·Developmental Biology·S B ScholnickM Rotenberg
Jun 1, 1990·Brain Research. Molecular Brain Research·U J Kang, T H Joh
Mar 1, 1993·Brain Research. Molecular Brain Research·A Le Van ThaiM J Weber
Feb 1, 1996·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·K S Bossinakou, E G Fragoulis
Jun 1, 1991·Mechanisms of Development·A N BillinS J Poole
Sep 6, 2003·Trends in Genetics : TIG·Patricia J WittkoppArtyom Kopp
Jul 18, 2012·Proceedings of the National Academy of Sciences of the United States of America·Huimin DengQili Feng
Jul 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·L L Wallrath, T B Friedman
May 1, 1996·Insect Molecular Biology·M T FerdigB M Christensen
Dec 8, 2005·Annual Review of Entomology·Ross B Hodgetts, Sandra L O'Keefe
Jun 23, 2011·Annual Review of Neuroscience·Nuria Flames, Oliver Hobert
Jan 2, 2013·Progress in Neuro-psychopharmacology & Biological Psychiatry·Bi ZhuChongde Lin
Oct 20, 2009·Cell·Adam Claridge-ChangGero Miesenböck
Nov 22, 2016·Bioscience, Biotechnology, and Biochemistry·Dai TsukiokaTakeshi Adachi
May 20, 1999·Microscopy Research and Technique·P H Taghert
Dec 22, 1995·The Journal of Biological Chemistry·G CharronJ P Julien
Feb 1, 1993·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Toshihiro Kitamoto, Paul M Salvaterra
Aug 1, 1995·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·A N Billin, S J Poole
Jan 15, 2021·Nature Reviews. Genetics·Evgeny Z KvonZeba Wunderlich
Aug 29, 2003·Development·Ilya Ruvinsky, Gary Ruvkun
Oct 29, 1997·Molecular and Cellular Biology·A E UvS J Bray
Dec 1, 1992·Molecular and Cellular Biology·G S Mastick, S B Scholnick

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