A neutral loss activation method for improved phosphopeptide sequence analysis by quadrupole ion trap mass spectrometry

Analytical Chemistry
Melanie J SchroederJoshua J Coon

Abstract

Recent advances in phosphopeptide enrichment prior to mass spectrometric analysis show genuine promise for characterization of phosphoproteomes. Tandem mass spectrometry of phosphopeptide ions, using collision-activated dissociation (CAD), often produces product ions dominated by the neutral loss of phosphoric acid. Here we describe a novel method, termed Pseudo MS(n), for phosphopeptide ion dissociation in quadrupole ion trap mass spectrometers. The method induces collisional activation of product ions, those resulting from neutral loss(es) of phosphoric acid, following activation of the precursor ion. Thus, the principal neutral loss product ions are converted into a variety of structurally informative species. Since product ions from both the original precursor activation and all subsequent neutral loss product activations are simultaneously stored, the method generates a "composite" spectrum containing fragments derived from multiple precursors. In comparison to analysis by conventional MS/MS (CAD), Pseudo MS(n) shows improved phosphopeptide ion dissociation for 7 out of 10 synthetic phosphopeptides, as judged by an automated search algorithm (TurboSEQUEST). A similar overall improvement was observed upon application of Pse...Continue Reading

References

Aug 1, 1974·Biomedical Mass Spectrometry·P Roepstorff, K Kristiansen
Nov 1, 1984·Biomedical Mass Spectrometry·P Roepstorff, J Fohlman
Oct 30, 1998·Journal of the American Society for Mass Spectrometry·J P DeGnore, J Qin
Jan 27, 2000·Cell·T Hunter
Jun 8, 2001·Annual Review of Biochemistry·M MannA Pandey
Oct 2, 2001·Current Opinion in Chemical Biology·D T McLachlin, B T Chait
Dec 26, 2001·Chemical Reviews·Z ChenM H Cobb
Mar 5, 2002·Nature Biotechnology·Scott B FicarroForest M White
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Michael J MacCossJohn R Yates
Nov 21, 2002·Journal of Mass Spectrometry : JMS·A TholeyW D Lehmann
Jan 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·Arthur R SalomonEric C Peters
Mar 1, 2003·Nature Biotechnology·Matthias Mann, Ole N Jensen
Jun 5, 2003·Journal of the American Society for Mass Spectrometry·Susanne C MoyerRobert J Cotter
Dec 13, 2003·Analytical Chemistry·Derek T McLachlin, Brian T Chait

❮ Previous
Next ❯

Citations

Aug 26, 2006·Analytical and Bioanalytical Chemistry·Peter SchubertJuergen Kast
Jul 24, 2012·Amino Acids·Luisa Beltran, Pedro R Cutillas
Apr 8, 2011·Journal of the American Society for Mass Spectrometry·Chih-Wei Liu, Chien-Chen Lai
Apr 8, 2011·Journal of the American Society for Mass Spectrometry·Hannes Hahne, Bernhard Kuster
Mar 25, 2008·Journal of the American Society for Mass Spectrometry·Pegah R Jalili, Haydn L Ball
Mar 3, 2009·Journal of the American Society for Mass Spectrometry·Grady R BlackenFrantisek Turecek
Apr 7, 2009·Journal of the American Society for Mass Spectrometry·Mingkun FuHilkka I Kenttämaa
Jul 1, 2006·Analytical Chemistry·Can BruceErol E Gulcicek
Sep 2, 2006·Analytical Chemistry·Grady R BlackenFrantisek Turecek
Apr 15, 2009·Analytical Chemistry·Joshua J Coon
Jul 10, 2012·Biochemistry·Janusz J PetkowskiIan G Macara
Feb 27, 2013·Chemical Reviews·Yaoyang ZhangJohn R Yates
May 3, 2008·Journal of Proteome Research·Cristian I RuseJohn R Yates
Aug 14, 2008·Journal of Proteome Research·Kristie L RoseJuan Ausió
Dec 17, 2008·Journal of Proteome Research·Peter J UlintzAlexey I Nesvizhskii
Oct 22, 2008·Nature Methods·Danielle L SwaneyJoshua J Coon
May 13, 2010·Nature Reviews. Molecular Cell Biology·Chunaram Choudhary, Matthias Mann
Jan 15, 2013·Nature Structural & Molecular Biology·Nicholas A LyonsDavid O Morgan
May 16, 2012·Proceedings of the National Academy of Sciences of the United States of America·Derek J BaileyJoshua J Coon
Apr 14, 2009·The Journal of Biological Chemistry·Paul J HurdTony Kouzarides
Apr 19, 2008·The Journal of Biological Chemistry·Michael P HousleyGerald W Hart
Dec 24, 2008·The Journal of Biological Chemistry·Michael P HousleyGerald W Hart
Aug 7, 2010·Molecular & Cellular Proteomics : MCP·Florian GnadMatthias Mann
Aug 28, 2010·Molecular & Cellular Proteomics : MCP·Emily H HallDavid L Brautigan
Aug 23, 2011·Molecular & Cellular Proteomics : MCP·Karin Grosstessner-HainKarl Mechtler
Feb 22, 2012·Molecular & Cellular Proteomics : MCP·Giuseppe PalmisanoMartin Røssel Larsen
Apr 13, 2012·Molecular & Cellular Proteomics : MCP·Mirita Franz-WachtelBoris Macek
May 31, 2008·Molecular & Cellular Proteomics : MCP·Alexander SchmidtBruno Domon

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Sean A BeausoleilSteven P Gygi
Proceedings of the National Academy of Sciences of the United States of America
John E P SykaDonald F Hunt
© 2022 Meta ULC. All rights reserved