A neutralizing monoclonal antibody to respiratory syncytial virus which binds to both F1 and F2 components of the fusion protein

The Journal of General Virology
A C SamsonG L Toms

Abstract

A virus-neutralizing monoclonal antibody (1E3) specifically immunoprecipitated the 70000 mol. wt. (70K) fusion (F) protein from respiratory syncytial (RS) virus-infected HeLa cells. Western blotting analysis of polypeptides from such cells separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions revealed that 1E3 was peculiar in that it bound to both F1 (50K) and F2 (20K) components of the F protein. Antibody subsequently eluted from either the F1 or the F2 regions of immunoblots re-bound to both F1 and F2 regions of the SDS-PAGE blot. These results show that monoclonal antibody 1E3 reacts with an epitope which is found on both F1 and F2 subunits of RS virus fusion protein.

Citations

Jan 1, 1990·Archives of Virology·M M WillcocksC R Madeley
Jan 1, 1990·Archives of Virology·T G Kimman, F Westenbrink
Mar 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S P FusseyS J Yeaman
Feb 13, 2021·Scientific Reports·Kristina BorochovaRudolf Valenta

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