A new 99mTc-red blood cell labeling procedure for cardiac blood pool imaging: clinical results

European Journal of Nuclear Medicine
H KelbaekS L Nielsen

Abstract

The first clinical results of a new 99mTc-red blood cell labeling procedure avoiding cell centrifugation are presented. One ml heparinized blood samples were incubated with small amounts of a stannous kit. By titration studies, ideal quantities of sodium hypochlorite for oxidation of extracellular tin and of EDTA as stabilizer of the label were found. The Cl- concentration and pH of the labeled blood were acceptable, and EDTA increased labeling yield and stability determined in vitro by a few percent. The new procedure gave a slightly higher labeling yield than a current technique using centrifugation of cells. Labeling efficiency expressed as cell bound/total activity was 96.6% +/- 1.3% in healthy subjects and 95.5% +/- 2.2% in cardiac patients and remained high for 2 h after reinjection. The biological halflife of labeled cells following the new procedure was 11-12 h rendering it suitable for serial determinations of radionuclide cardiography.

References

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Citations

Oct 1, 1996·Seminars in Nuclear Medicine·J C HungR J Hammes
Mar 1, 1990·Progress in Cardiovascular Diseases·H Kelbaek
Jan 1, 1990·International Journal of Radiation Applications and Instrumentation. Part B, Nuclear Medicine and Biology·A ProulxK Y Gulenchyn
Aug 26, 2014·Journal of Endodontics·Shuichi HashimotoKatsuhisa Sunada
Jan 1, 1990·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·N Fogh-AndersenO Siggaard-Andersen
Apr 1, 1991·Scandinavian Journal of Clinical and Laboratory Investigation·J K ThomsenA Devantier

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