A new acid-fast trichrome stain for simultaneous detection of Cryptosporidium parvum and microsporidial species in stool specimens.

Journal of Clinical Microbiology
R IgnatiusJ Wagner

Abstract

The detection in stool specimens of Cryptosporidium parvum and microsporidia, the most frequent parasitic pathogens causing diarrhea in AIDS patients, until now has depended on two different staining methods. However, since double infections occur and minimization of laboratory costs is mandatory, development of a method for simultaneous detection of these parasites appeared desirable. We report on a new, inexpensive, and easy-to-perform staining procedure to demonstrate both acid-fast oocysts of C. parvum and other coccidia, as well as microsporidial spores. This acid-fast trichrome stain yields results comparable to those obtained by the Kinyoun and modified trichrome methods and considerably reduces the time necessary for microscopic examination.

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Citations

Jun 23, 1999·Clinical Pediatrics·S P NelsonF Gonzalez-Crussi
Sep 18, 2012·The Pediatric Infectious Disease Journal·Mahmoud AgholiMohammad Hossein Motazedian
Feb 24, 2015·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Lerine Bahy El-Dine El-ShazlySally Mohammed Fouad
Nov 17, 2017·Clinical Microbiology Reviews·Lynne S GarciaGovinda Visvesvara
Jun 5, 2002·Revista Da Sociedade Brasileira De Medicina Tropical·Carla R Rigo, Regina Maura B Franco
Jan 20, 2018·Parasitology Research·Shahira A Ahmed, Panagiotis Karanis
Mar 2, 2018·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Elham Kord-SarkachiMoluok Beiromvand
Apr 27, 2000·Archives of Pathology & Laboratory Medicine·B S Reisner, J Spring

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