A New Anaesthetic Protocol for Adult Zebrafish (Danio rerio): Propofol Combined with Lidocaine

PloS One
A M ValentimL M Antunes

Abstract

The increasing use of zebrafish model has not been accompanied by the evolution of proper anaesthesia for this species in research. The most used anaesthetic in fishes, MS222, may induce aversion, reduction of heart rate, and consequently high mortality, especially during long exposures. Therefore, we aim to explore new anaesthetic protocols to be used in zebrafish by studying the quality of anaesthesia and recovery induced by different concentrations of propofol alone and in combination with different concentrations of lidocaine. In experiment A, eighty-three AB zebrafish were randomly assigned to 7 different groups: control, 2.5 (2.5P), 5 (5P) or 7.5 μg/ml (7.5P) of propofol; and 2.5 μg/ml of propofol combined with 50, (P/50L), 100 (P/100L) or 150 μg/ml (P/150L) of lidocaine. Zebrafish were placed in an anaesthetic water bath and time to lose the equilibrium, reflex to touch, reflex to a tail pinch, and respiratory rate were measured. Time to gain equilibrium was also assessed in a clean tank. Five and 24 hours after anaesthesia recovery, zebrafish were evaluated concerning activity and reactivity. Afterwards, in a second phase of experiments (experiment B), the best protocol of the experiment A was compared with a new group ...Continue Reading

References

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Citations

Dec 3, 2016·Laboratory Animals·Tânia MartinsLuis M Antunes
Jun 13, 2019·Zebrafish·Ophelia EhrlichPatricia Regina Jusuf
Jan 31, 2020·Journal of Applied Toxicology : JAT·Lin HeShan Zheng
Dec 6, 2018·Laboratory Animals·Tânia MartinsLuis Marques Antunes
Aug 5, 2017·Scientific Reports·Gareth D ReadmanJoanna C Murrell
Mar 6, 2021·PloS One·Jennifer P Owen, Robert N Kelsh
Apr 27, 2021·Frontiers in Neuroscience·Nils OhnesorgeLars Lewejohann
Jun 3, 2021·International Journal of Molecular Sciences·Ditte Gry EllmanDitte Caroline Andersen

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Methods Mentioned

BETA
sedation

Software Mentioned

SPSS
GraphPad Prism

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