A new and efficient culture method for porcine bone marrow-derived M1- and M2-polarized macrophages

Veterinary Immunology and Immunopathology
Jiye GaoHenk P Haagsman

Abstract

Macrophages play an important role in the innate immune system as part of the mononuclear phagocyte system (MPS). They have a pro-inflammatory signature (M1-polarized macrophages) or anti-inflammatory signature (M2-polarized macrophages) based on expression of surface receptors and secretion of cytokines. However, very little is known about the culture of macrophages from pigs and more specific about the M1 and M2 polarization in vitro. Porcine monocytes or mononuclear bone marrow cells were used to culture M1- and M2-polarized macrophages in the presence of GM-CSF and M-CSF, respectively. Surface receptor expression was measured with flow cytometry and ELISA was used to quantify cytokine secretion in response to LPS and PAM3CSK4 stimulation. Human monocyte-derived macrophages were used as control. Porcine M1- and M2-polarized macrophages were cultured best using porcine GM-CSF and murine M-CSF, respectively. Cultures from bone marrow cells resulted in a higher yield M1- and M2-polarized macrophages which were better comparable to human monocyte-derived macrophages than cultures from porcine monocytes. Porcine M1-polarized macrophages displayed the characteristic fried egg shape morphology, lower CD163 expression and low IL-10 ...Continue Reading

References

Sep 1, 1968·The Journal of Experimental Medicine·R van Furth, Z A Cohn
Feb 24, 2001·Nature·M KristiansenS K Moestrup
Jan 4, 2003·Nature Reviews. Immunology·Siamon Gordon
Sep 1, 1962·The Journal of Experimental Medicine·G B MACKANESS
Apr 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Frank A W VerreckTom H M Ottenhoff
May 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Geert RaesSilvano Sozzani
Dec 3, 2005·Nature Reviews. Immunology·Siamon Gordon, Philip R Taylor
Mar 3, 2007·Journal of Leukocyte Biology·Markus Schneemann, Gabriele Schoeden
Mar 27, 2007·International Journal of Biological Sciences·Joan K Lunney
Jun 8, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Kendall P MyersGregory C Gray
Jan 22, 2008·The Journal of Nutrition·Michael E Spurlock, Nicholas K Gabler
Mar 28, 2008·The Journal of Infectious Diseases·Eileen Thacker, Bruce Janke
Mar 15, 2008·Nature Reviews. Immunology·Hajime Kono, Kenneth L Rock
Jun 20, 2008·Nature Immunology·Carl Nathan
Jul 22, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nigel D L SavageTom H M Ottenhoff
Oct 1, 2008·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Mark J Truty, Rory L Smoot
Nov 5, 2008·The Journal of Clinical Investigation·Sharon D RicardoAllison A Eddy
Nov 26, 2008·Nature Reviews. Immunology·David M Mosser, Justin P Edwards
Mar 21, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Marzia LeidiJosée Golay
Dec 10, 2009·Proceedings of the National Academy of Sciences of the United States of America·Andrew Mehle, Jennifer A Doudna
Jul 29, 2010·Seminars in Liver Disease·Thomas A Wynn, Luke Barron
Dec 14, 2011·Trends in Microbiology·François MeurensVolker Gerdts
Mar 7, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ronan KapetanovicDavid A Hume
Apr 27, 2013·Nature·Thomas A WynnJeffrey W Pollard
Nov 28, 2013·Veterinary Immunology and Immunopathology·Susan M KnetterAmanda E Ramer-Tait
Mar 19, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Elena Sierra-FilardiAngel L Corbí
May 20, 2014·Immunity·Alexander Mildner, Steffen Jung
Jul 19, 2014·Immunity·Slava EpelmanGwendalyn J Randolph
Oct 17, 2014·Immunological Reviews·Rebecca GentekMichael H Sieweke
Mar 1, 2012·Transboundary and Emerging Diseases·S Nuntawan Na AyudhyaR Thanawongnuwech
Apr 12, 2015·Annual Review of Immunology·Chen VarolSteffen Jung
Apr 15, 2015·American Journal of Respiratory Cell and Molecular Biology·Abdullah A TariqueEmmanuelle Fantino

❮ Previous
Next ❯

Citations

Jul 6, 2019·Frontiers in Immunology·Noushin LotfiNafiseh Esmaeil
Jun 10, 2021·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Megan VierhoutKjetil Ask

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.