A new anticancer toxin based on HER2/neu-specific DARPin and photoactive flavoprotein miniSOG

Biochimie
G M ProshkinaS M Deyev

Abstract

Cytotoxic effects of a new targeted phototoxin DARPin-miniSOG and mechanism of its action were investigated in vitro. It was determined that DARPin-miniSOG causes light-induced death of HER2/neu-positive cancer cells (IC50 0.8 μM). Treatment of the cells with DARPin-miniSOG in the presence of ascorbic acid eliminated the light-induced cytotoxic action of the protein. This observation suggests the involvement of oxidative stress in the mechanism of the phototoxin action. DNA fragmentation analysis, caspase-3 activity assay and PI-staining of HER2/neu-positive cancer cells treated with DARPin-miniSOG indicated that phototoxin induces necrotic cell death under blue light illumination. Co-localization analysis showed that DARPin-miniSOG accumulates mostly in endosomes and lysosomes.

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Citations

Mar 5, 2016·Cancer Investigation·Zhihao ZhangXiaoyan Zhao
May 15, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Evgeniya SokolovaSergey Deyev
Aug 17, 2016·BioTechniques·Alina P RyuminaKonstantin A Lukyanov
Sep 9, 2018·Proceedings of the National Academy of Sciences of the United States of America·Evgenii L GuryevSergey M Deyev
Jul 21, 2017·Doklady. Biochemistry and Biophysics·E I ShramovaR V Petrov
Sep 1, 2017·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Olga N ShilovaSergey M Deyev
Nov 10, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Suman DasDhermendra K Tiwari
Oct 31, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Salvador GomezZhiwei Hu
Jun 3, 2021·International Journal of Molecular Sciences·Olga ShilovaSergey Deyev
Jul 10, 2018·Chemical Reviews·Aba LosiAndreas Möglich
Nov 4, 2020·Biomacromolecules·Heinz Fabian RaberFrank Rosenau
Sep 8, 2021·Cancer Cell International·Reza MahmoudiMohammad Rahmati

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