PMID: 9448206Feb 3, 1998Paper

A new application of BCG antibody for rapid screening of various tissue microorganisms

Journal of the American Academy of Dermatology
Heinz KutznerH Hügel

Abstract

In routine dermatopathology there is growing demand for a simple, fast, cost-effective, and highly sensitive screening tool for the detection of microorganisms. Our purpose was to determine whether immunostaining with polyclonal anti-Mycobacterium bovis (BCG), which is known for its interspecies cross-reactivity, is a suitable screening method for many common microorganisms in dermatopathologic specimens. A total of 254 formalin-fixed, paraffin-embedded skin specimens of viral, protozoal, fungal, and bacterial infections were stained with appropriate histochemical stains and with anti-BCG. Anti-BCG labeled bacteria and fungi with high sensitivity and minimal background staining, but did not react with spirochetes, viruses, or protozoa (Leishmania). The quality and sensitivity of anti-BCG staining were superior to conventional histochemical stains. Because of its cross-reactivity with many bacteria and fungi as well as its high sensitivity and minimal background staining, the anti-BCG immunostain is a promising screening tool for the detection of the most common bacterial and fungal microorganisms in paraffin-embedded skin specimens.

Citations

Nov 2, 2004·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·F MeierC Scherwitz
Jun 29, 1999·Clinics in Dermatology·D R MehreganD A Mehregan
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Aug 21, 2001·Journal of the American Academy of Dermatology·L Requena, E Sánchez Yus

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