A new approach for activation of the kiwifruit cysteine protease for usage in in-vitro testing.

Molecular Biology Reports
Andrijana NešićMarija Gavrović-Jankulović

Abstract

Actinidin (Act d 1), a highly abundant cysteine protease from kiwifruit, is one of the major contributors to the development of kiwifruit allergy. Many studies have focused on the optimization of Act d 1 purification and its role in the development of food allergies. Testing on cell culture monolayers is a common step in the elucidation of food allergen sensitization. In the case of cysteine proteases, an additional activation step with L-cysteine is required before the testing. Hence, we aimed to evaluate whether L-cysteine already present in commonly used cell culture media would suffice for Act d 1 activation. Successfully activated Act d 1 (98.1% of proteolytic activity, as compared to L-cysteine activated Act d 1) was further tested in two commonly used 2D model systems (Caco-2 and HEK293 cells) to evaluate its role on the mRNA expression of cytokines involved in the innate immunity (IL-1β, IL-6, TNFα, TSLP). Furthermore, the contribution of Act d 1 in the promotion of inflammation through regulation of inducible nitric oxide synthase (iNOS) mRNA expression was also examined. These results demonstrate that activation of cysteine proteases can be achieved without previous enzyme incubation in L-cysteine -containing solution...Continue Reading

References

Jan 1, 1976·Methods in Enzymology·G R Drapeau
May 9, 1998·The Journal of Allergy and Clinical Immunology·E A PastorelloC Ortolani
Mar 17, 2001·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·C RusznakS Lozewicz
Jun 22, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·P A RecheJ F Bazan
Aug 23, 2001·International Immunopharmacology·J W Coleman
Jan 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·Hai-Chon Lee, Steven F Ziegler
Feb 28, 2008·Nature Reviews. Immunology·Hamida Hammad, Bart N Lambrecht
Nov 1, 2011·Plant Physiology·Niels J NieuwenhuizenRoss G Atkinson
Jan 10, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Milica GrozdanovicMarija Gavrovic-Jankulovic
Nov 13, 2012·The Journal of Allergy and Clinical Immunology·Thuy-My LeAndré C Knulst
Feb 12, 2013·Advances in Food and Nutrition Research·Mike Boland
Mar 19, 2014·Journal of the Science of Food and Agriculture·Milica M GrozdanovicMarija Gavrovic-Jankulovic
Jul 22, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Milena CavicMarija Gavrovic-Jankulovic
Feb 3, 2015·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Francesca UbertiPatrizia Restani
Feb 5, 2015·Current Opinion in Gastroenterology·Elisa SchiaviLiam O'Mahony
Dec 25, 2015·Biochimica Et Biophysica Acta·Milica M GrozdanovicMarija Gavrovic-Jankulovic
Jul 4, 2016·The Journal of Allergy and Clinical Immunology·Claire GalandRaif S Geha
Nov 1, 2016·Nature Reviews. Immunology·Wong YuKari C Nadeau
Jan 13, 2017·Clinical and Translational Allergy·Emek KocatürkClive Grattan
Nov 1, 2015·Revue Française D'allergologie·Eva Untersmayr
Jan 9, 2018·The Journal of Allergy and Clinical Immunology·Hugh A SampsonCezmi A Akdis

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