A new approach for quantifying epigenetic landscapes.

The Journal of Biological Chemistry
Wolfgang Fischle

Abstract

ChIP-Seq is a widespread experimental method for determining the global enrichment of chromatin modifications and genome-associated factors. Whereas it is straightforward to compare the relative genomic distribution of these epigenetic features, researchers have also made efforts to compare their signal strength using external references for normalization. New work now suggests that these "spike-ins" could lead to inaccurate conclusions due to intrinsic issues of the methodology and instead calls for new criteria of experimental reporting that may permit internal standardization when certain parameters are fulfilled.

References

Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M J Solomon, A Varshavsky
Jun 2, 2007·Science·David S JohnsonBarbara Wold
Dec 2, 2014·Cell Reports·David A OrlandoMatthew G Guenther
Dec 30, 2015·Molecular and Cellular Biology·Kaifu ChenJessica K Tyler
Apr 20, 2018·Nucleic Acids Research·Michael J GuertinAndrew N Holding
Oct 1, 2020·The Journal of Biological Chemistry·Bradley M DicksonScott B Rothbart

❮ Previous
Next ❯

Citations

Jun 30, 2021·Journal of Leukocyte Biology·Lingyu ZhaoHanjie Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.