PMID: 11604171Oct 18, 2001Paper

A new approach for weed control in a cucurbit field employing an attenuated potyvirus-vector for herbicide resistance

Journal of Biotechnology
Y M ShibolethA Gal-On

Abstract

Expression of bar, a phosphinothricin acetyltransferase, in plant tissues, leads to resistance of these plants to glufosinate ammonium based herbicides. We have created a bar expressing, attenuated zucchini yellow mosaic potyvirus-vector, AGII-Bar, to enable herbicide use in cucurbit fields. The parental vector, ZYMV-AGII, has been rendered environmentally safe by both disease-symptom attenuation and aphid-assisted virus transmission abolishment. The recombinant AGII-Bar virus-encoding cDNA, when inoculated on diverse cucurbits was highly infectious, accumulated to similar levels as AGII, and elicited attenuated AGII-like symptoms. Potted cucurbits inoculated with AGII-Bar became herbicide resistant about a week post-inoculation. Herbicide resistance was sustained in squash over a period of at least 26 days and for at least 60 days in cucumber grown in a net-house under commercial conditions. To test the applicability of AGII-Bar use in a weed-infested field, a controlled experiment including more than 450 plants inoculated with this construct, was performed. Different dosages of glufosinate ammonium were sprayed, 2 weeks after planting, on the foliage of melons, cucumbers, squash, and watermelons. AGII-Bar provided protection ...Continue Reading

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Citations

Jul 31, 2002·Annual Review of Phytopathology·Gregory P PogueWayne P Fitzmaurice
Mar 7, 2008·Critical Reviews in Biotechnology·Hector G Nuñez-PaleniusDaniel J Cantliffe

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