A new approach to the quantitative measurement of dense LDL subfractions

Nutrition, Metabolism, and Cardiovascular Diseases : NMCD
Gabriela BergLaura Schreier

Abstract

Small dense low-density lipoproteins (LDLs) should be considered a major risk factor for cardiovascular disease, but there is still no recommended method for measuring them or expressing clinical values. We measured the dense LDL portion relatively simply by isolating it using density ultracentrifugation and then giving it a relative, quantitative value. Dense LDLs (d=1.048-1.063 g/mL) were isolated from human plasma at the same time as total LDL (d=1.021-1.063 g/mL) by means of sequential ultracentrifugation, and the former was assessed as a percentage of the latter. A receiver operator characteristic (ROC) curve was used to compare the different LDL components as markers of dense LDLs. The proposed method was compared with non-denaturing gradient gel electrophoresis (NDGGE). In order to obtain clinical data, the dense LDL portion was measured in diabetic and postmenopausal subjects and healthy controls. The ROC curve showed that cholesterol level was a more accurate marker of dense LDLs. The within-run precision (CV) was 2.28%, and the between-run CV was 5.1%. Analytical recovery was 80.2+/-1.6%. The correlation between the proposed method and NDGGE was r=0.90, p<0.001. The dense LDL percentage significantly correlated with s...Continue Reading

References

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Citations

Jul 24, 2012·The Journal of Pediatrics·Matthew BensonNelly Mauras
Apr 17, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·M L MuzzioL Schreier
Apr 28, 2012·Diabetes/metabolism Research and Reviews·V MiksztowiczG Berg
Sep 27, 2005·Climacteric : the Journal of the International Menopause Society
Oct 23, 2008·Metabolism: Clinical and Experimental·Verónica MiksztowiczGabriela Berg
Apr 14, 2017·Diabetology & Metabolic Syndrome·Marília Izar Helfenstein FonsecaSandra Roberta G Ferreira

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