A new approach using micro HPLC-MS/MS for multi-mycotoxin analysis in maize samples

Mycotoxin Research
Sebastian HickertHans-Ulrich Humpf

Abstract

Using micro high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) a simple and fast method for the quantitative determination of 26 mycotoxins was developed. Sample preparation consists of a single extraction step and a dilute-and-shoot approach without further cleanup. With a total run time of 9 min and solvent consumption below 0.3 mL per chromatographic run, the presented method is cost-effective. All toxins regulated by the European Commission with maximum or guidance levels in grain products (fumonisins B1 and B2 (FB1 and FB2)); deoxynivalenol (DON); aflatoxins B1, G1, B2, and G2 (AFB1, AFG1, AFB2, and AFG2); ochratoxin A (OTA); T-2 and HT-2 toxins; and zearalenone (ZEN) can be quantified with this method. Furthermore, the enniatins B, B1, A, and A1 (EnB, EnB1, EnA, and EnA1); beauvericin (BEA); 3-acetyl-deoxynivalenol (3-AcDON); fusarin C (FusC); sterigmatocystin (STC); gliotoxin (GT); and the Alternaria toxins alternariol (AOH), alternariol monomethyl ether (AME), altenuene (ALT), tentoxin (TEN), and altertoxin I (ATX I) can also be quantified. For all regulated compounds, recoveries ranged between 76 and 120%. For all other toxins, the recovery was at least 51%. The method was applied for the anal...Continue Reading

References

Mar 8, 2006·Molecular Nutrition & Food Research·Michael BretzHans-Ulrich Humpf
Sep 21, 2007·Journal of Agricultural and Food Chemistry·Benedikt CramerHans-Ulrich Humpf
Mar 19, 2008·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Martien C SpanjerJos M Scholten
Jul 1, 2008·Journal of Agricultural and Food Chemistry·Benedikt CramerHans-Ulrich Humpf
Dec 10, 2009·Journal of Agricultural and Food Chemistry·Sofie MonbaliuSarah De Saeger
Mar 10, 2010·Analytical and Bioanalytical Chemistry·R R RasmussenK F Nielsen
Nov 1, 2004·Molecular Plant Pathology·Rubella S Goswami, H Corby Kistler
May 15, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Souheib OueslatiJosé Luis Martínez Vidal
Feb 1, 2012·Mycotoxin Research·Florian HübnerHans-Ulrich Humpf

❮ Previous
Next ❯

Citations

Oct 17, 2015·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Alberto Biancardi, Chiara Dall'Asta
Oct 31, 2015·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Elaine MarleySusan Macdonald
Sep 19, 2015·Journal of Chromatography. a·Carlos E D NazarioFernando M Lanças
Apr 26, 2016·Journal of Proteomics·Tamara MartinovićDjuro Josić
May 6, 2016·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Jeremy R SchwartzbordDan L Brown
Oct 22, 2016·Analytical and Bioanalytical Chemistry·Terence HetzelTorsten C Schmidt
Sep 7, 2016·Rapid Communications in Mass Spectrometry : RCM·Sebastian HickertHans-Ulrich Humpf
May 17, 2018·Analytical and Bioanalytical Chemistry·Hannes PuntscherBenedikt Warth
Mar 24, 2018·Journal of Food and Drug Analysis·Yu-Yan YuTong-Ying Wang
Mar 17, 2019·Analytical and Bioanalytical Chemistry·Pengqi GuoPeng Li
Jun 18, 2020·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Minghui LiSha Li
Jul 30, 2017·Mycotoxin Research·Sebastian HickertHans-Ulrich Humpf
May 2, 2017·Journal of Agricultural and Food Chemistry·Henning Sören SchmidtHans-Ulrich Humpf

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
Souheib OueslatiJosé Luis Martínez Vidal
Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
F SoleimanyA Khatib
Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
Josep RubertCarla Soler
© 2022 Meta ULC. All rights reserved