A New Automated Method for the Analysis of Aromatic Amines in Human Urine by GC-MS/MS

Journal of Analytical Toxicology
Shrila MazumderTiffany H Seyler

Abstract

Cigarette smoking significantly increases the risk of cancer and cardiovascular diseases as well as premature death. Aromatic amines (AAs) such as o-toluidine, 2-aminonaphthalene and 4-aminobiphenyl are found in cigarette smoke and are well-established human bladder carcinogens presumably acting via the formation of DNA adducts. These amines may be metabolized in the liver to acetylated or glucuronidated forms or oxidized to a hydroxylamine which may react with protein and DNA to form adducts. Free, acetylated and glucuronidated AAs are excreted in urine and can be measured as exposure biomarkers. Using isotope dilution GC-MS/MS, our laboratory quantifies six urinary AAs that are known or suspected carcinogens-o-toluidine, 2,6-dimethylaniline, o-anisidine, 1-aminonaphthalene, 2-aminonaphthalene and 4-aminobiphenyl-for large population studies such as the National Health and Nutrition Examination Survey (NHANES). We also monitor two additional corresponding structural isomers-2-aminobiphenyl and 3-aminobiphenyl-to verify isomer separation. A new and improved automated sample preparation method was developed to quantify these AAs, in which, sample cleanup was done via Supported Liquid Extraction (SLE+ ISOLUTE®) on a Hamilton STAR...Continue Reading

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Citations

Aug 28, 2020·Analytical Methods : Advancing Methods and Applications·Arezoo HabibagahiCariton Kubwabo
Jan 10, 2020·Journal of Clinical and Translational Hepatology·Cyriac Abby PhilipsPhilip Augustine
Aug 16, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Sridhar Chinthakindi, Kurunthachalam Kannan

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