A new basophil activation test using CD63 and CCR3 in allergy to antibiotics

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
B EberleinJ Ring

Abstract

Flow cytometric basophil activation tests (BAT) have been developed as cellular tests for in vitro diagnosis of IgE-mediated reactions. Different markers and techniques have been used after stimulation with various allergens. It was the aim of the present study to compare an established BAT (Flow-CAST) with a newly developed basophil activation protocol using CD63 and CCR3 (Flow2 CAST) in patients with type-I allergy to antibiotics. Twenty-four patients with a history of type-I allergy to antibiotics were examined. A careful allergy history was taken, and skin tests and determination of specific IgE antibodies were performed. Two different BAT using CD63 expression but different protocols were carried out after stimulation with different concentrations of antibiotics. Fifteen healthy subjects without a history of antibiotic allergy were studied as controls. The Flow2 CAST showed a higher sensitivity than the Flow-CAST (55% vs. 53%) with regard to patients' history. Specificity was 80% both for the Flow2 CAST and for the Flow-CAST with regard to controls with negative history and negative RAST. These results show the value of two different BAT as cellular tests in the in vitro diagnosis of patients with antibiotic allergy with e...Continue Reading

References

Aug 1, 1993·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·P W EwanP J Lachmann
Feb 16, 2002·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Guillaume MonneretJacques Bienvenu
Apr 4, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M J TorresM Blanca
Apr 4, 2002·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M L SanzA L De Weck
Sep 27, 2003·Allergy·M J TorresUNKNOWN EAACI Interest Group on Drug Hypersensitivity
Nov 17, 2004·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M J TorresM Blanca
Dec 31, 2004·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·S M ErdmannH F Merk
Jul 14, 2006·International Archives of Allergy and Immunology·Jörg Kleine-TebbeBernhard F Gibbs
Feb 13, 2008·International Archives of Allergy and Immunology·A L de WeckJ Sainte-Laudy
Mar 12, 2008·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·N AbuafR Girot
Apr 17, 2008·Cytometry. Part B, Clinical Cytometry·D G EboW J Stevens
Jul 1, 2008·The American Journal of Medicine·Anne B Yates

❮ Previous
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Citations

Sep 30, 2010·Der Hautarzt; Zeitschrift für Dermatologie, Venerologie, und verwandte Gebiete·B Wedi, A Kapp
Nov 29, 2012·Current Allergy and Asthma Reports·Emily C McGowan, Sarbjit Saini
Oct 19, 2012·International Archives of Allergy and Immunology·Heidi R MyrsetMaaike M B W Dooper
Aug 21, 2012·American Journal of Respiratory Cell and Molecular Biology·Sebastian OederJeroen T M Buters
Feb 7, 2014·Allergy, Asthma, and Clinical Immunology : Official Journal of the Canadian Society of Allergy and Clinical Immunology·Natalia HagauCristina Petrisor
Nov 22, 2013·Asia Pacific Allergy·Woo-Jung Song, Yoon-Seok Chang
May 21, 2011·Expert Review of Clinical Immunology·Julie LeysenDidier G Ebo
Sep 17, 2014·Expert Review of Clinical Immunology·Astrid P UyttebroekDidier G Ebo
May 3, 2011·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Panwas PinnobphunJettanong Klaewsongkram
Jun 8, 2011·Allergy·D G EboI Terreehorst
Nov 24, 2011·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·C H KatelarisP Wark
Jun 19, 2010·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·G Monneret
Nov 18, 2014·Cytometry. Part B, Clinical Cytometry·Bernadette EberleinJeroen Buters
Apr 11, 2014·Human Vaccines & Immunotherapeutics·Salvatore Chirumbolo
Apr 19, 2016·Journal of Agricultural and Food Chemistry·Katrin Franz-OberdorfWilfried G Schwab
Feb 27, 2016·The Journal of Allergy and Clinical Immunology·Friedrich HorakAlbert Yu
Jul 15, 2016·Current Allergy and Asthma Reports·Hans Jürgen HoffmannDonald MacGlashan
Jan 25, 2012·International Archives of Allergy and Immunology·Salvatore Chirumbolo
Jul 3, 2013·International Archives of Allergy and Immunology·Heidi R MyrsetMaaike M B W Dooper
Apr 5, 2017·Expert Review of Clinical Pharmacology·Tahia D FernandezMaria I Montañez
Jun 8, 2017·International Journal of Molecular Sciences·Cristobalina MayorgaMaria J Torres
Oct 10, 2018·Minerva pediatrica·Mara De AmiciGianluigi Marseglia
Jun 6, 2017·Immunity, Inflammation and Disease·Bernadette EberleinUlf Darsow
Mar 11, 2017·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Anne-Emmanuelle Depince-BergerClaude Lambert
Nov 9, 2017·American Journal of Industrial Medicine·Rolf MergetThomas Brüning
Nov 16, 2018·Current Allergy and Asthma Reports·Oliver HemmingsAlexandra F Santos
Mar 13, 2019·Current Allergy and Asthma Reports·Lucila CamposPedro Giavina-Bianchi
Feb 7, 2021·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Marsus PumarCelia Zubrinich
Nov 13, 2020·Asia Pacific Allergy·Yuttana SrinoulprasertDuangjit Kanistanon
Nov 17, 2020·Allergy, Asthma & Immunology Research·Dan Xuan ZhuJi Fu Wei
Oct 16, 2021·Journal of Anesthesia·Tomonori TakazawaUNKNOWN Working Group for the Preparation of Practical Guidelines for the Response to Anaphylaxis, Safety Committee of the Japanese

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