PMID: 3749363Apr 1, 1986Paper

A new biosensor for rapid BOD estimation by using immobilized growing cell beads

Proceedings of the National Science Council, Republic of China. Part B, Life Sciences
Y C SuM L Liu

Abstract

A closed, reactor-type sensor system for rapid estimation of BOD by the use of immobilized growing whole cells of a facultative bacterium, Bacillus polymyxa D-21, in kappa-carrageenan and an oxygen electrode is described. This system consists of a transformer, a recorder, a thermostated water bath (30 +/- 1 degree C), a magnetic stirrer (200 rpm), an oxygen electrode, and a flask containing 10 g (wet weight) immobilized cell beads of 2-3 mm in diameter. The total time required for an assay is less than 15 min. The calibration curves obtained show a linear relationship between the rate of oxygen consumption and the concentration of standard wastewater solution which contains equal amounts of glucose and L-glutamic acid each at a concentration below 10 mg/l. The measurement is independent of the pH of the wastewater solution under investigation. The rate of oxygen consumption was reproducible with an average relative error of 5.89% when a standard wastewater solution containing 12 mg/l of glucose and 12 mg/l of L-glutamic acid was assayed 54 times. When the immobilized cell beads were suspended in a 0.1 M phosphate buffer (pH 6.0) and stored at 25 degrees C, the response of the sensor system remained unchanged for about 30 days. ...Continue Reading

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